Visualization of pig sperm chromosomes by in-vitro penetration of zona-free hamster ova.

P. Creighton; J. A. Houghton

doi:10.1530/jrf.0.0800619

Visualization of pig sperm chromosomes by in-vitro penetration of zona-free hamster ova.

P. Creighton, J. A. Houghton

Infectious Disease

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

17 Citations (Scopus)

Abstract

Zona-free hamster eggs were penetrated by pig spermatozoa capacitated using bovine follicular fluid and Percoll gradients. A mean penetration rate of 80.1% was obtained from 5 ejaculates from 2 boars. Penetrated eggs were cultured and analysable metaphase chromosome spreads were obtained from 16.8%. The analysis of 20 pig sperm complements indicated that 9 were 19, Y, 10 were 19, X and 1 appeared to have an XY sex chromosome constitution.

Original language	English
Pages (from-to)	619-622
Number of pages	4
Journal	Journal of Reproduction and Fertility
Volume	80
Issue number	2
DOIs	https://doi.org/10.1530/jrf.0.0800619
Publication status	Published - Jul 1987

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10.1530/jrf.0.0800619

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abstract = "Zona-free hamster eggs were penetrated by pig spermatozoa capacitated using bovine follicular fluid and Percoll gradients. A mean penetration rate of 80.1% was obtained from 5 ejaculates from 2 boars. Penetrated eggs were cultured and analysable metaphase chromosome spreads were obtained from 16.8%. The analysis of 20 pig sperm complements indicated that 9 were 19, Y, 10 were 19, X and 1 appeared to have an XY sex chromosome constitution.",

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AB - Zona-free hamster eggs were penetrated by pig spermatozoa capacitated using bovine follicular fluid and Percoll gradients. A mean penetration rate of 80.1% was obtained from 5 ejaculates from 2 boars. Penetrated eggs were cultured and analysable metaphase chromosome spreads were obtained from 16.8%. The analysis of 20 pig sperm complements indicated that 9 were 19, Y, 10 were 19, X and 1 appeared to have an XY sex chromosome constitution.

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Visualization of pig sperm chromosomes by in-vitro penetration of zona-free hamster ova.

Abstract

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