Use of a tetrazolium based colorimetric assay in assessing photoradiation therapy in vitro

A. P. McHaLe; L. McHaLe

doi:10.1016/0304-3835(88)90293-5

Use of a tetrazolium based colorimetric assay in assessing photoradiation therapy in vitro

A. P. McHaLe, L. McHaLe

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

111 Citations (Scopus)

Abstract

The suitability of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT)] assay to the determination of cell viability following photoradiation therapy (PRT) of human breast and melanoma cell lines has been examined. Results have been shown to correlate with those obtained using a clonogenic assay system. Using the MTT assay system it was shown that differences occur in the susceptibility of both lines to PDT. In addition it has been demonstrated that both lines differ with respect to their ability to develop photosensitivity in the presence of hematoporphyrin derivative (HpD). In the absence of serum this difference is not as obvious. This MTT assay provides a valid, simple and semi-automatable system for assessment of PRT in vitro.

Original language	English
Pages (from-to)	315-321
Number of pages	7
Journal	Cancer Letters
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(88)90293-5
Publication status	Published - 30 Aug 1988
Externally published	Yes

Keywords

Breast tumour
HeNe laser
Hematoporphyrin derivative
MTT assay
Melanoma
Photoradiation therapy

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10.1016/0304-3835(88)90293-5

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title = "Use of a tetrazolium based colorimetric assay in assessing photoradiation therapy in vitro",

abstract = "The suitability of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT)] assay to the determination of cell viability following photoradiation therapy (PRT) of human breast and melanoma cell lines has been examined. Results have been shown to correlate with those obtained using a clonogenic assay system. Using the MTT assay system it was shown that differences occur in the susceptibility of both lines to PDT. In addition it has been demonstrated that both lines differ with respect to their ability to develop photosensitivity in the presence of hematoporphyrin derivative (HpD). In the absence of serum this difference is not as obvious. This MTT assay provides a valid, simple and semi-automatable system for assessment of PRT in vitro.",

keywords = "Breast tumour, HeNe laser, Hematoporphyrin derivative, MTT assay, Melanoma, Photoradiation therapy",

author = "McHaLe, \{A. P.\} and L. McHaLe",

year = "1988",

month = aug,

day = "30",

doi = "10.1016/0304-3835(88)90293-5",

language = "English",

volume = "41",

pages = "315--321",

journal = "Cancer Letters",

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number = "3",

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TY - JOUR

T1 - Use of a tetrazolium based colorimetric assay in assessing photoradiation therapy in vitro

AU - McHaLe, A. P.

AU - McHaLe, L.

PY - 1988/8/30

Y1 - 1988/8/30

N2 - The suitability of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT)] assay to the determination of cell viability following photoradiation therapy (PRT) of human breast and melanoma cell lines has been examined. Results have been shown to correlate with those obtained using a clonogenic assay system. Using the MTT assay system it was shown that differences occur in the susceptibility of both lines to PDT. In addition it has been demonstrated that both lines differ with respect to their ability to develop photosensitivity in the presence of hematoporphyrin derivative (HpD). In the absence of serum this difference is not as obvious. This MTT assay provides a valid, simple and semi-automatable system for assessment of PRT in vitro.

AB - The suitability of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT)] assay to the determination of cell viability following photoradiation therapy (PRT) of human breast and melanoma cell lines has been examined. Results have been shown to correlate with those obtained using a clonogenic assay system. Using the MTT assay system it was shown that differences occur in the susceptibility of both lines to PDT. In addition it has been demonstrated that both lines differ with respect to their ability to develop photosensitivity in the presence of hematoporphyrin derivative (HpD). In the absence of serum this difference is not as obvious. This MTT assay provides a valid, simple and semi-automatable system for assessment of PRT in vitro.

KW - Breast tumour

KW - HeNe laser

KW - Hematoporphyrin derivative

KW - MTT assay

KW - Melanoma

KW - Photoradiation therapy

UR - https://www.scopus.com/pages/publications/0023764985

U2 - 10.1016/0304-3835(88)90293-5

DO - 10.1016/0304-3835(88)90293-5

M3 - Article

SN - 0304-3835

VL - 41

SP - 315

EP - 321

JO - Cancer Letters

JF - Cancer Letters

IS - 3

ER -

Use of a tetrazolium based colorimetric assay in assessing photoradiation therapy in vitro

Abstract

Keywords

UN SDGs

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