Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier

Keith Dowling; Sam C.W. Hyde; Nicholas P. Barry; Christopher Dainty; Paul M.W. French; Alun J. Hughes; M. J. Lever; Anthony K.L. Dymoke-Bradshaw; Jonathan D. Hares; Paul A. Kellett

doi:10.1117/12.273522

Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier

Keith Dowling, Sam C.W. Hyde, Nicholas P. Barry, Christopher Dainty, Paul M.W. French, Alun J. Hughes, M. J. Lever, Anthony K.L. Dymoke-Bradshaw, Jonathan D. Hares, Paul A. Kellett

Research output: Contribution to a Journal (Peer & Non Peer) › Conference article › peer-review

1 Citation (Scopus)

Abstract

We report the demonstration of a high temporal resolution fluorescence lifetime imaging (FLIM) system using a time- gated image intensifier to provide whole field FLIM images. The gate width has been optimized to 110 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences of 20 ps, have been detected. Environmental changes of the fluorescent indicator, Lucifer Yellow, have been sensed by measuring changes in its fluorescence lifetime when unbound and when bound to the protein albumin.

Original language	English
Pages (from-to)	20-23
Number of pages	4
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	2980
DOIs	https://doi.org/10.1117/12.273522
Publication status	Published - 1997
Externally published	Yes
Event	Advances in Fluorescence Sensing Technology III - San Jose, CA, United States Duration: 9 Feb 1997 → 9 Feb 1997

Keywords

All solid state lasers
Fluorescence lifetime imaging
Functional imaging
Gated image intensifier
Medical imaging

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10.1117/12.273522

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Dowling, K., Hyde, S. C. W., Barry, N. P., Dainty, C., French, P. M. W., Hughes, A. J., Lever, M. J., Dymoke-Bradshaw, A. K. L., Hares, J. D., & Kellett, P. A. (1997). Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier. Proceedings of SPIE - The International Society for Optical Engineering, 2980, 20-23. https://doi.org/10.1117/12.273522

@article{af6c8c928eab493592c12d83ce2d435d,

title = "Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier",

abstract = "We report the demonstration of a high temporal resolution fluorescence lifetime imaging (FLIM) system using a time- gated image intensifier to provide whole field FLIM images. The gate width has been optimized to 110 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences of 20 ps, have been detected. Environmental changes of the fluorescent indicator, Lucifer Yellow, have been sensed by measuring changes in its fluorescence lifetime when unbound and when bound to the protein albumin.",

keywords = "All solid state lasers, Fluorescence lifetime imaging, Functional imaging, Gated image intensifier, Medical imaging",

author = "Keith Dowling and Hyde, \{Sam C.W.\} and Barry, \{Nicholas P.\} and Christopher Dainty and French, \{Paul M.W.\} and Hughes, \{Alun J.\} and Lever, \{M. J.\} and Dymoke-Bradshaw, \{Anthony K.L.\} and Hares, \{Jonathan D.\} and Kellett, \{Paul A.\}",

year = "1997",

doi = "10.1117/12.273522",

language = "English",

volume = "2980",

pages = "20--23",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Advances in Fluorescence Sensing Technology III ; Conference date: 09-02-1997 Through 09-02-1997",

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TY - JOUR

T1 - Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier

AU - Dowling, Keith

AU - Hyde, Sam C.W.

AU - Barry, Nicholas P.

AU - Dainty, Christopher

AU - French, Paul M.W.

AU - Hughes, Alun J.

AU - Lever, M. J.

AU - Dymoke-Bradshaw, Anthony K.L.

AU - Hares, Jonathan D.

AU - Kellett, Paul A.

PY - 1997

Y1 - 1997

N2 - We report the demonstration of a high temporal resolution fluorescence lifetime imaging (FLIM) system using a time- gated image intensifier to provide whole field FLIM images. The gate width has been optimized to 110 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences of 20 ps, have been detected. Environmental changes of the fluorescent indicator, Lucifer Yellow, have been sensed by measuring changes in its fluorescence lifetime when unbound and when bound to the protein albumin.

AB - We report the demonstration of a high temporal resolution fluorescence lifetime imaging (FLIM) system using a time- gated image intensifier to provide whole field FLIM images. The gate width has been optimized to 110 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences of 20 ps, have been detected. Environmental changes of the fluorescent indicator, Lucifer Yellow, have been sensed by measuring changes in its fluorescence lifetime when unbound and when bound to the protein albumin.

KW - All solid state lasers

KW - Fluorescence lifetime imaging

KW - Functional imaging

KW - Gated image intensifier

KW - Medical imaging

UR - https://www.scopus.com/pages/publications/7344233339

U2 - 10.1117/12.273522

DO - 10.1117/12.273522

M3 - Conference article

AN - SCOPUS:7344233339

SN - 0277-786X

VL - 2980

SP - 20

EP - 23

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Advances in Fluorescence Sensing Technology III

Y2 - 9 February 1997 through 9 February 1997

ER -

Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier

Abstract

Keywords

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