TY - JOUR
T1 - Transcriptional regulation of expression of the rainbow trout albumin gene by estrogen
AU - Flouriot, G.
AU - Ducouret, B.
AU - Byrnes, L.
AU - Valotaire, Y.
PY - 1998/6
Y1 - 1998/6
N2 - Estrogens modulate the expression of many liver-specific genes in oviparous species. For instance, expression of the estrogen receptor and vitellogenin genes is strongly up-regulated by estradiol in rainbow trout liver. Using hepatocyte primary cultures, we demonstrate that trout albumin (Alb) gene is also regulated by this hormone. Indeed, treatment of hepatocytes with 1 μM estradiol led, after 24 h, to a dramatic decrease in Alb mRNA level. To investigate the mechanism of this down-regulation, run- off experiments were performed and mRNA half-lives were determined in the presence and absence of estradiol. The results show that the down-regulation of Alb mRNA expression by estrogens occurs only at the transcriptional level.
AB - Estrogens modulate the expression of many liver-specific genes in oviparous species. For instance, expression of the estrogen receptor and vitellogenin genes is strongly up-regulated by estradiol in rainbow trout liver. Using hepatocyte primary cultures, we demonstrate that trout albumin (Alb) gene is also regulated by this hormone. Indeed, treatment of hepatocytes with 1 μM estradiol led, after 24 h, to a dramatic decrease in Alb mRNA level. To investigate the mechanism of this down-regulation, run- off experiments were performed and mRNA half-lives were determined in the presence and absence of estradiol. The results show that the down-regulation of Alb mRNA expression by estrogens occurs only at the transcriptional level.
UR - http://www.scopus.com/inward/record.url?scp=0031863867&partnerID=8YFLogxK
U2 - 10.1677/jme.0.0200355
DO - 10.1677/jme.0.0200355
M3 - Article
C2 - 9687158
AN - SCOPUS:0031863867
SN - 0952-5041
VL - 20
SP - 355
EP - 362
JO - Journal of Molecular Endocrinology
JF - Journal of Molecular Endocrinology
IS - 3
ER -
