Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes

Thomas Schwarzl; Andrea Keogh; Georgina Shaw; Aleksandar Krstic; Elizabeth Clayton; Desmond G. Higgins; Walter Kolch; Frank Barry

doi:10.1038/s41597-023-02686-y

Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes

Thomas Schwarzl, Andrea Keogh, Georgina Shaw, Aleksandar Krstic, Elizabeth Clayton, Desmond G. Higgins, Walter Kolch, Frank Barry

Medicine

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

9 Citations (Scopus)

Abstract

Articular cartilage has only very limited regenerative capacities in humans. Tissue engineering techniques for cartilage damage repair are limited in the production of hyaline cartilage. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells and can be differentiated into mature cartilage cells, chondrocytes, which could be used for repairing damaged cartilage. Chondrogenesis is a highly complex, relatively inefficient process lasting over 3 weeks in vitro. Methods: In order to better understand chondrogenic differentiation, especially the commitment phase, we have performed transcriptional profiling of MSC differentiation into chondrocytes from early timepoints starting 15 minutes after induction to 16 hours and fully differentiated chondrocytes at 21 days in triplicates.

Original language	English
Article number	758
Journal	Scientific data
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41597-023-02686-y
Publication status	Published - Dec 2023

Access to Document

10.1038/s41597-023-02686-y

Cite this

@article{42b0be96b31f4b1594ece01fe3645695,

title = "Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes",

abstract = "Articular cartilage has only very limited regenerative capacities in humans. Tissue engineering techniques for cartilage damage repair are limited in the production of hyaline cartilage. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells and can be differentiated into mature cartilage cells, chondrocytes, which could be used for repairing damaged cartilage. Chondrogenesis is a highly complex, relatively inefficient process lasting over 3 weeks in vitro. Methods: In order to better understand chondrogenic differentiation, especially the commitment phase, we have performed transcriptional profiling of MSC differentiation into chondrocytes from early timepoints starting 15 minutes after induction to 16 hours and fully differentiated chondrocytes at 21 days in triplicates.",

author = "Thomas Schwarzl and Andrea Keogh and Georgina Shaw and Aleksandar Krstic and Elizabeth Clayton and Higgins, \{Desmond G.\} and Walter Kolch and Frank Barry",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

month = dec,

doi = "10.1038/s41597-023-02686-y",

language = "English",

volume = "10",

journal = "Scientific data",

issn = "2052-4463",

publisher = "Nature Research",

number = "1",

}

TY - JOUR

T1 - Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes

AU - Schwarzl, Thomas

AU - Keogh, Andrea

AU - Shaw, Georgina

AU - Krstic, Aleksandar

AU - Clayton, Elizabeth

AU - Higgins, Desmond G.

AU - Kolch, Walter

AU - Barry, Frank

PY - 2023/12

Y1 - 2023/12

N2 - Articular cartilage has only very limited regenerative capacities in humans. Tissue engineering techniques for cartilage damage repair are limited in the production of hyaline cartilage. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells and can be differentiated into mature cartilage cells, chondrocytes, which could be used for repairing damaged cartilage. Chondrogenesis is a highly complex, relatively inefficient process lasting over 3 weeks in vitro. Methods: In order to better understand chondrogenic differentiation, especially the commitment phase, we have performed transcriptional profiling of MSC differentiation into chondrocytes from early timepoints starting 15 minutes after induction to 16 hours and fully differentiated chondrocytes at 21 days in triplicates.

AB - Articular cartilage has only very limited regenerative capacities in humans. Tissue engineering techniques for cartilage damage repair are limited in the production of hyaline cartilage. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells and can be differentiated into mature cartilage cells, chondrocytes, which could be used for repairing damaged cartilage. Chondrogenesis is a highly complex, relatively inefficient process lasting over 3 weeks in vitro. Methods: In order to better understand chondrogenic differentiation, especially the commitment phase, we have performed transcriptional profiling of MSC differentiation into chondrocytes from early timepoints starting 15 minutes after induction to 16 hours and fully differentiated chondrocytes at 21 days in triplicates.

UR - https://www.scopus.com/pages/publications/85175708332

U2 - 10.1038/s41597-023-02686-y

DO - 10.1038/s41597-023-02686-y

M3 - Article

C2 - 37923731

AN - SCOPUS:85175708332

SN - 2052-4463

VL - 10

JO - Scientific data

JF - Scientific data

IS - 1

M1 - 758

ER -

Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes

Abstract

Access to Document

Other files and links

Fingerprint

Cite this