Transcription factor AP2 controls cnidarian germ cell induction

Timothy Q. DuBuc; Christine E. Schnitzler; Eleni Chrysostomou; Emma T. McMahon; Febrimarsa; James M. Gahan; Tara Buggie; Sebastian G. Gornik; Shirley Hanley; Sofia N. Barreira; Paul Gonzalez; Andreas D. Baxevanis; Uri Frank

doi:10.1126/science.aay6782

Transcription factor AP2 controls cnidarian germ cell induction

Timothy Q. DuBuc, Christine E. Schnitzler, Eleni Chrysostomou, Emma T. McMahon, Febrimarsa, James M. Gahan, Tara Buggie, Sebastian G. Gornik, Shirley Hanley, Sofia N. Barreira, Paul Gonzalez, Andreas D. Baxevanis, Uri Frank

Molecular Biosciences

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

49 Citations (Scopus)

Abstract

Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How germ fate is induced in these animals, and whether this process is related to bilaterian embryonic germline induction, is unknown. We show that transcription factor AP2 (Tfap2), a regulator of mammalian germ lines, acts to commit adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian Hydractinia symbiolongicarpus. Tfap2 mutants lacked germ cells and gonads. Transplanted wild-type cells rescued gonad development but not germ cell induction in Tfap2 mutants. Forced expression of Tfap2 in i-cells converted them to germ cells. Therefore, Tfap2 is a regulator of germ cell commitment across germ line–sequestering and germ line–nonsequestering animals.

Original language	English
Pages (from-to)	757-762
Number of pages	6
Journal	Science
Volume	367
Issue number	6479
DOIs	https://doi.org/10.1126/science.aay6782
Publication status	Published - 14 Feb 2020

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title = "Transcription factor AP2 controls cnidarian germ cell induction",

abstract = "Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How germ fate is induced in these animals, and whether this process is related to bilaterian embryonic germline induction, is unknown. We show that transcription factor AP2 (Tfap2), a regulator of mammalian germ lines, acts to commit adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian Hydractinia symbiolongicarpus. Tfap2 mutants lacked germ cells and gonads. Transplanted wild-type cells rescued gonad development but not germ cell induction in Tfap2 mutants. Forced expression of Tfap2 in i-cells converted them to germ cells. Therefore, Tfap2 is a regulator of germ cell commitment across germ line–sequestering and germ line–nonsequestering animals.",

author = "DuBuc, \{Timothy Q.\} and Schnitzler, \{Christine E.\} and Eleni Chrysostomou and McMahon, \{Emma T.\} and Febrimarsa and Gahan, \{James M.\} and Tara Buggie and Gornik, \{Sebastian G.\} and Shirley Hanley and Barreira, \{Sofia N.\} and Paul Gonzalez and Baxevanis, \{Andreas D.\} and Uri Frank",

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doi = "10.1126/science.aay6782",

language = "English",

volume = "367",

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journal = "Science",

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T1 - Transcription factor AP2 controls cnidarian germ cell induction

AU - DuBuc, Timothy Q.

AU - Schnitzler, Christine E.

AU - Chrysostomou, Eleni

AU - McMahon, Emma T.

AU - Febrimarsa,

AU - Gahan, James M.

AU - Buggie, Tara

AU - Gornik, Sebastian G.

AU - Hanley, Shirley

AU - Barreira, Sofia N.

AU - Gonzalez, Paul

AU - Baxevanis, Andreas D.

AU - Frank, Uri

PY - 2020/2/14

Y1 - 2020/2/14

N2 - Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How germ fate is induced in these animals, and whether this process is related to bilaterian embryonic germline induction, is unknown. We show that transcription factor AP2 (Tfap2), a regulator of mammalian germ lines, acts to commit adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian Hydractinia symbiolongicarpus. Tfap2 mutants lacked germ cells and gonads. Transplanted wild-type cells rescued gonad development but not germ cell induction in Tfap2 mutants. Forced expression of Tfap2 in i-cells converted them to germ cells. Therefore, Tfap2 is a regulator of germ cell commitment across germ line–sequestering and germ line–nonsequestering animals.

AB - Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How germ fate is induced in these animals, and whether this process is related to bilaterian embryonic germline induction, is unknown. We show that transcription factor AP2 (Tfap2), a regulator of mammalian germ lines, acts to commit adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian Hydractinia symbiolongicarpus. Tfap2 mutants lacked germ cells and gonads. Transplanted wild-type cells rescued gonad development but not germ cell induction in Tfap2 mutants. Forced expression of Tfap2 in i-cells converted them to germ cells. Therefore, Tfap2 is a regulator of germ cell commitment across germ line–sequestering and germ line–nonsequestering animals.

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DO - 10.1126/science.aay6782

M3 - Article

SN - 0036-8075

VL - 367

SP - 757

EP - 762

JO - Science

JF - Science

IS - 6479

ER -

Transcription factor AP2 controls cnidarian germ cell induction

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