Three-dimensional structural view of the central metabolic network of thermotoga maritima

Ying Zhang; Ines Thiele; Dana Weekes; Zhanwen Li; Lukasz Jaroszewski; Krzysztof Ginalski; Ashley M. Deacon; John Wooley; Scott A. Lesley; Ian A. Wilson; Bernhard Palsson; Andrei Osterman; Adam Godzik

doi:10.1126/science.1174671

Three-dimensional structural view of the central metabolic network of thermotoga maritima

Ying Zhang, Ines Thiele, Dana Weekes, Zhanwen Li, Lukasz Jaroszewski, Krzysztof Ginalski, Ashley M. Deacon, John Wooley, Scott A. Lesley, Ian A. Wilson, Bernhard Palsson, Andrei Osterman, Adam Godzik

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

154 Citations (Scopus)

Abstract

Metabolie pathways have traditionally been described in terms of biochemical reactions and metabolites. With the use of structural genomics and systems biology, we generated a three-dimensional reconstruction of the central metabolic network of the bacterium Thermotoga maritima. The network encompassed 478 proteins, of which 120 were determined by experiment and 358 were modeled. Structural analysis revealed that proteins forming the network are dominated by a small number (only 182) of basic shapes (folds) performing diverse but mostly related functions. Most of these folds are already present in the essential core (∼30%) of the network, and its expansion by nonessential proteins is achieved with relatively few additional folds. Thus, integration of structural data with networks analysis generates insight into the function, mechanism, and evolution of biological networks.

Original language	English
Pages (from-to)	1544-1549
Number of pages	6
Journal	Science
Volume	325
Issue number	5947
DOIs	https://doi.org/10.1126/science.1174671
Publication status	Published - 2009
Externally published	Yes

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title = "Three-dimensional structural view of the central metabolic network of thermotoga maritima",

abstract = "Metabolie pathways have traditionally been described in terms of biochemical reactions and metabolites. With the use of structural genomics and systems biology, we generated a three-dimensional reconstruction of the central metabolic network of the bacterium Thermotoga maritima. The network encompassed 478 proteins, of which 120 were determined by experiment and 358 were modeled. Structural analysis revealed that proteins forming the network are dominated by a small number (only 182) of basic shapes (folds) performing diverse but mostly related functions. Most of these folds are already present in the essential core (∼30\%) of the network, and its expansion by nonessential proteins is achieved with relatively few additional folds. Thus, integration of structural data with networks analysis generates insight into the function, mechanism, and evolution of biological networks.",

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doi = "10.1126/science.1174671",

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T1 - Three-dimensional structural view of the central metabolic network of thermotoga maritima

AU - Zhang, Ying

AU - Thiele, Ines

AU - Weekes, Dana

AU - Li, Zhanwen

AU - Jaroszewski, Lukasz

AU - Ginalski, Krzysztof

AU - Deacon, Ashley M.

AU - Wooley, John

AU - Lesley, Scott A.

AU - Wilson, Ian A.

AU - Palsson, Bernhard

AU - Osterman, Andrei

AU - Godzik, Adam

PY - 2009

Y1 - 2009

N2 - Metabolie pathways have traditionally been described in terms of biochemical reactions and metabolites. With the use of structural genomics and systems biology, we generated a three-dimensional reconstruction of the central metabolic network of the bacterium Thermotoga maritima. The network encompassed 478 proteins, of which 120 were determined by experiment and 358 were modeled. Structural analysis revealed that proteins forming the network are dominated by a small number (only 182) of basic shapes (folds) performing diverse but mostly related functions. Most of these folds are already present in the essential core (∼30%) of the network, and its expansion by nonessential proteins is achieved with relatively few additional folds. Thus, integration of structural data with networks analysis generates insight into the function, mechanism, and evolution of biological networks.

AB - Metabolie pathways have traditionally been described in terms of biochemical reactions and metabolites. With the use of structural genomics and systems biology, we generated a three-dimensional reconstruction of the central metabolic network of the bacterium Thermotoga maritima. The network encompassed 478 proteins, of which 120 were determined by experiment and 358 were modeled. Structural analysis revealed that proteins forming the network are dominated by a small number (only 182) of basic shapes (folds) performing diverse but mostly related functions. Most of these folds are already present in the essential core (∼30%) of the network, and its expansion by nonessential proteins is achieved with relatively few additional folds. Thus, integration of structural data with networks analysis generates insight into the function, mechanism, and evolution of biological networks.

UR - https://www.scopus.com/pages/publications/70349345898

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DO - 10.1126/science.1174671

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VL - 325

SP - 1544

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JO - Science

JF - Science

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ER -

Three-dimensional structural view of the central metabolic network of thermotoga maritima

Abstract

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