Thiol proteases released in vitro by Fasciola hepatica

John P. Dalton; Mary Heffernan

doi:10.1016/0166-6851(89)90118-7

Thiol proteases released in vitro by Fasciola hepatica

John P. Dalton
, Mary Heffernan

Dublin City University

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

140 Citations (Scopus)

Abstract

Immature Fasciola hepatica release 11 distinct proteases when cultured in vitro for 16 h as revealed by gelatin-substrate sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Six of these proteases are active in the pH range 4.5 to 8.0. Five are acid proteases, being most active in the pH range 3.0 to 4.5. The majority of proteases released in vitro by immature flukes are also released by mature flukes; however, a 40-kDa protease released by immature flukes is a very minor protease released by mature flukes. The activity of all proteases is inhibited by leupeptin, l-trans-epoxysuccinyl-leucylamido(4-guanidino)butane, phenylmethylsulfonyl fluoride and iodoacetamide and enhanced or stabilized by the reducing agents cysteine and dithiothreitol. Therefore, all F. hepatica in vitro-released proteases identified by gelatin-substrate SDS-PAGE are thiol proteases.

Original language	English
Pages (from-to)	161-166
Number of pages	6
Journal	Molecular and Biochemical Parasitology
Volume	35
Issue number	2
DOIs	https://doi.org/10.1016/0166-6851(89)90118-7
Publication status	Published - 15 Jun 1989
Externally published	Yes

Keywords

Fasciola hepatica
In vitro released product
Protease inhibitor
Thiol protease
Trematode

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10.1016/0166-6851(89)90118-7

Cite this

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title = "Thiol proteases released in vitro by Fasciola hepatica",

abstract = "Immature Fasciola hepatica release 11 distinct proteases when cultured in vitro for 16 h as revealed by gelatin-substrate sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Six of these proteases are active in the pH range 4.5 to 8.0. Five are acid proteases, being most active in the pH range 3.0 to 4.5. The majority of proteases released in vitro by immature flukes are also released by mature flukes; however, a 40-kDa protease released by immature flukes is a very minor protease released by mature flukes. The activity of all proteases is inhibited by leupeptin, l-trans-epoxysuccinyl-leucylamido(4-guanidino)butane, phenylmethylsulfonyl fluoride and iodoacetamide and enhanced or stabilized by the reducing agents cysteine and dithiothreitol. Therefore, all F. hepatica in vitro-released proteases identified by gelatin-substrate SDS-PAGE are thiol proteases.",

keywords = "Fasciola hepatica, In vitro released product, Protease inhibitor, Thiol protease, Trematode",

author = "Dalton, \{John P.\} and Mary Heffernan",

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doi = "10.1016/0166-6851(89)90118-7",

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journal = "Molecular and Biochemical Parasitology",

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}

TY - JOUR

T1 - Thiol proteases released in vitro by Fasciola hepatica

AU - Dalton, John P.

AU - Heffernan, Mary

PY - 1989/6/15

Y1 - 1989/6/15

N2 - Immature Fasciola hepatica release 11 distinct proteases when cultured in vitro for 16 h as revealed by gelatin-substrate sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Six of these proteases are active in the pH range 4.5 to 8.0. Five are acid proteases, being most active in the pH range 3.0 to 4.5. The majority of proteases released in vitro by immature flukes are also released by mature flukes; however, a 40-kDa protease released by immature flukes is a very minor protease released by mature flukes. The activity of all proteases is inhibited by leupeptin, l-trans-epoxysuccinyl-leucylamido(4-guanidino)butane, phenylmethylsulfonyl fluoride and iodoacetamide and enhanced or stabilized by the reducing agents cysteine and dithiothreitol. Therefore, all F. hepatica in vitro-released proteases identified by gelatin-substrate SDS-PAGE are thiol proteases.

AB - Immature Fasciola hepatica release 11 distinct proteases when cultured in vitro for 16 h as revealed by gelatin-substrate sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Six of these proteases are active in the pH range 4.5 to 8.0. Five are acid proteases, being most active in the pH range 3.0 to 4.5. The majority of proteases released in vitro by immature flukes are also released by mature flukes; however, a 40-kDa protease released by immature flukes is a very minor protease released by mature flukes. The activity of all proteases is inhibited by leupeptin, l-trans-epoxysuccinyl-leucylamido(4-guanidino)butane, phenylmethylsulfonyl fluoride and iodoacetamide and enhanced or stabilized by the reducing agents cysteine and dithiothreitol. Therefore, all F. hepatica in vitro-released proteases identified by gelatin-substrate SDS-PAGE are thiol proteases.

KW - Fasciola hepatica

KW - In vitro released product

KW - Protease inhibitor

KW - Thiol protease

KW - Trematode

UR - https://www.scopus.com/pages/publications/0024410978

U2 - 10.1016/0166-6851(89)90118-7

DO - 10.1016/0166-6851(89)90118-7

M3 - Article

C2 - 2770786

AN - SCOPUS:0024410978

SN - 0166-6851

VL - 35

SP - 161

EP - 166

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 2

ER -

Thiol proteases released in vitro by Fasciola hepatica

Abstract

Keywords

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