The structure of the O-polysaccharide of the Pseudoalteromonas rubra ATCC 29570T lipopolysaccharide containing a keto sugar

Michelle Kilcoyne; Alexander S. Shashkov; Yuriy A. Knirel; Raisa P. Gorshkova; Evgeny L. Nazarenko; Elena P. Ivanova; Natalya M. Gorshkova; Sof'ya N. Senchenkova; Angela V. Savage

doi:10.1016/j.carres.2005.07.007

The structure of the O-polysaccharide of the Pseudoalteromonas rubra ATCC 29570^T lipopolysaccharide containing a keto sugar

Michelle Kilcoyne
, Alexander S. Shashkov
, Yuriy A. Knirel
, Raisa P. Gorshkova
, Evgeny L. Nazarenko
, Elena P. Ivanova
, Natalya M. Gorshkova
, Sof'ya N. Senchenkova
, Angela V. Savage

Russian Academy of Sciences
Far E. Br. of the Russ. Acad. of S.

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

15 Citations (Scopus)

Abstract

The structure of the phenol-soluble polysaccharide from Pseudoalteromonas rubra type strain ATCC 29570^T has been elucidated using ¹H and ¹³C NMR spectroscopy, including 2D COSY, TOCSY, gNOESY, ROESY, ¹H,¹³C gHMQC and gHMBC experiments. It is concluded that the trisaccharide repeating unit of the polysaccharide has the following structure: →4)-α-L-GalpNAm3AcA-(1→3)-α-Sugp-(1→4)- β-D-GlcpNAc3NAcylA-(1→ where Sug is 2-acetamido-2,6-dideoxy-d-xylo- hexos-4-ulose, Am is acetimidoyl and Acyl is a malic acid residue, which is O-acetylated in ∼70% of the units.

Original language	English
Pages (from-to)	2369-2375
Number of pages	7
Journal	Carbohydrate Research
Volume	340
Issue number	15
DOIs	https://doi.org/10.1016/j.carres.2005.07.007
Publication status	Published - 31 Oct 2005

Keywords

2-Acetamido-2,6-dideoxy-D-xylo-hexos-4-ulose
Malic acid
NMR spectroscopy
Polysaccharide
Pseudoalteromonas rubra

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10.1016/j.carres.2005.07.007

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Kilcoyne, M., Shashkov, A. S., Knirel, Y. A., Gorshkova, R. P., Nazarenko, E. L., Ivanova, E. P., Gorshkova, N. M., Senchenkova, S. N., & Savage, A. V. (2005). The structure of the O-polysaccharide of the Pseudoalteromonas rubra ATCC 29570^T lipopolysaccharide containing a keto sugar. Carbohydrate Research, 340(15), 2369-2375. https://doi.org/10.1016/j.carres.2005.07.007

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title = "The structure of the O-polysaccharide of the Pseudoalteromonas rubra ATCC 29570T lipopolysaccharide containing a keto sugar",

abstract = "The structure of the phenol-soluble polysaccharide from Pseudoalteromonas rubra type strain ATCC 29570T has been elucidated using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, gNOESY, ROESY, 1H,13C gHMQC and gHMBC experiments. It is concluded that the trisaccharide repeating unit of the polysaccharide has the following structure: →4)-α-L-GalpNAm3AcA-(1→3)-α-Sugp-(1→4)- β-D-GlcpNAc3NAcylA-(1→ where Sug is 2-acetamido-2,6-dideoxy-d-xylo- hexos-4-ulose, Am is acetimidoyl and Acyl is a malic acid residue, which is O-acetylated in ∼70\% of the units.",

keywords = "2-Acetamido-2,6-dideoxy-D-xylo-hexos-4-ulose, Malic acid, NMR spectroscopy, Polysaccharide, Pseudoalteromonas rubra",

author = "Michelle Kilcoyne and Shashkov, \{Alexander S.\} and Knirel, \{Yuriy A.\} and Gorshkova, \{Raisa P.\} and Nazarenko, \{Evgeny L.\} and Ivanova, \{Elena P.\} and Gorshkova, \{Natalya M.\} and Senchenkova, \{Sof'ya N.\} and Savage, \{Angela V.\}",

year = "2005",

month = oct,

day = "31",

doi = "10.1016/j.carres.2005.07.007",

language = "English",

volume = "340",

pages = "2369--2375",

journal = "Carbohydrate Research",

issn = "0008-6215",

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number = "15",

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T1 - The structure of the O-polysaccharide of the Pseudoalteromonas rubra ATCC 29570T lipopolysaccharide containing a keto sugar

AU - Kilcoyne, Michelle

AU - Shashkov, Alexander S.

AU - Knirel, Yuriy A.

AU - Gorshkova, Raisa P.

AU - Nazarenko, Evgeny L.

AU - Ivanova, Elena P.

AU - Gorshkova, Natalya M.

AU - Senchenkova, Sof'ya N.

AU - Savage, Angela V.

PY - 2005/10/31

Y1 - 2005/10/31

N2 - The structure of the phenol-soluble polysaccharide from Pseudoalteromonas rubra type strain ATCC 29570T has been elucidated using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, gNOESY, ROESY, 1H,13C gHMQC and gHMBC experiments. It is concluded that the trisaccharide repeating unit of the polysaccharide has the following structure: →4)-α-L-GalpNAm3AcA-(1→3)-α-Sugp-(1→4)- β-D-GlcpNAc3NAcylA-(1→ where Sug is 2-acetamido-2,6-dideoxy-d-xylo- hexos-4-ulose, Am is acetimidoyl and Acyl is a malic acid residue, which is O-acetylated in ∼70% of the units.

AB - The structure of the phenol-soluble polysaccharide from Pseudoalteromonas rubra type strain ATCC 29570T has been elucidated using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, gNOESY, ROESY, 1H,13C gHMQC and gHMBC experiments. It is concluded that the trisaccharide repeating unit of the polysaccharide has the following structure: →4)-α-L-GalpNAm3AcA-(1→3)-α-Sugp-(1→4)- β-D-GlcpNAc3NAcylA-(1→ where Sug is 2-acetamido-2,6-dideoxy-d-xylo- hexos-4-ulose, Am is acetimidoyl and Acyl is a malic acid residue, which is O-acetylated in ∼70% of the units.

KW - 2-Acetamido-2,6-dideoxy-D-xylo-hexos-4-ulose

KW - Malic acid

KW - NMR spectroscopy

KW - Polysaccharide

KW - Pseudoalteromonas rubra

UR - https://www.scopus.com/pages/publications/25444449333

U2 - 10.1016/j.carres.2005.07.007

DO - 10.1016/j.carres.2005.07.007

M3 - Article

SN - 0008-6215

VL - 340

SP - 2369

EP - 2375

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 15

ER -

The structure of the O-polysaccharide of the Pseudoalteromonas rubra ATCC 29570^T lipopolysaccharide containing a keto sugar

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