TY - JOUR
T1 - The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105)
AU - Barry, Frank P.
AU - Boynton, Raymond E.
AU - Haynesworth, Stephen
AU - Murphy, J. Mary
AU - Zaia, Joseph
PY - 1999/11/11
Y1 - 1999/11/11
N2 - Mesenchymal stem cells are multipotent cells resident in the bone marrow throughout adulthood which have the capacity to differentiate into cartilage, bone, fat, muscle, and tendon. A number of monoclonal antibodies raised against human MSCs have been shown to react with surface antigens on these cells in vitro. A protein of molecular mass 92 kDa was immunoprecipitated using the SH-2 monoclonal antibody. This was purified and identified by peptide sequencing analysis and mass spectrometry as endoglin (CD105), the TGF-β receptor III present on endothelial cells, syncytiotrophoblasts, macrophage, and connective tissue stromal cells. Endoglin on MSCs potentially plays a role in TGF-β signalling in the control of chondrogenic differentiation of MSCs and also in mediating interactions between MSCs and haematopoietic cells in the bone marrow microenvironment.
AB - Mesenchymal stem cells are multipotent cells resident in the bone marrow throughout adulthood which have the capacity to differentiate into cartilage, bone, fat, muscle, and tendon. A number of monoclonal antibodies raised against human MSCs have been shown to react with surface antigens on these cells in vitro. A protein of molecular mass 92 kDa was immunoprecipitated using the SH-2 monoclonal antibody. This was purified and identified by peptide sequencing analysis and mass spectrometry as endoglin (CD105), the TGF-β receptor III present on endothelial cells, syncytiotrophoblasts, macrophage, and connective tissue stromal cells. Endoglin on MSCs potentially plays a role in TGF-β signalling in the control of chondrogenic differentiation of MSCs and also in mediating interactions between MSCs and haematopoietic cells in the bone marrow microenvironment.
UR - http://www.scopus.com/inward/record.url?scp=0033547347&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.1620
DO - 10.1006/bbrc.1999.1620
M3 - Article
SN - 0006-291X
VL - 265
SP - 134
EP - 139
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
