The Isolation of scFvs Against Small Target Molecules

Iain Shaw; Marian Kane

doi:10.1007/978-1-60327-302-2_7

The Isolation of scFvs Against Small Target Molecules

Iain Shaw, Marian Kane

Molecular Biosciences

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

3 Citations (Scopus)

Abstract

Phage display has the capacity to rapidly isolate recombinant antibodies against protein targets and other molecules of significant size. However, there is no obvious lower limit to the power of the selection methods: this chapter describes how the techniques of phage display can be adapted to allow the isolation of antibodies against very small Compounds. Antibodies generated in this way have many uses including the detection and quantitative analysis of the target chemical moiety in samples such as foods, water, and body fluids.

Original language	English (Ireland)
Pages (from-to)	83-97
Number of pages	15
Journal	Methods Mol Biol
Volume	562
DOIs	https://doi.org/10.1007/978-1-60327-302-2_7
Publication status	Published - 1 Sep 2009

Authors (Note for portal: view the doc link for the full list of authors)

Authors
Shaw, I,Kane, M,Aitken, R

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10.1007/978-1-60327-302-2_7

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The Isolation of scFvs Against Small Target Molecules

Abstract

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