The formation of collagen type I fibrils in vitro

The assembly of collagen fibrils as a function of temperature and collagen concentration was studied. It was shown that temperature increases from 25 to 35°C, the degree of ordering of collagen fibrils increases 1.5-fold at collagen concentration above 1 mg/ml and 2-fold at low collagen concentration. A maximum ordering of fibril structure occurs under conditions close to physiological (T ≈ 35°C and collagen concentration 1.2 mg/ml). As temperature is elevated from 30 to 35°C, the packing of collagen molecules in fibrils becomes more ordered: the values of enthalpy and entropy of the transition of fibrils from the native to a disordered state decrease at all collagen concentrations used. At high collagen concentration, the dimentions of cooperative blocks in fibrils formed at 25 and 30°C coincide with those of cooperative blocks of monomeric collagen in solution. Upon increasing the temperature to 35°C, the dimensions of cooperative blocks increase.