TY - JOUR
T1 - The biophysical, biochemical, and biological toolbox for tenogenic phenotype maintenance in vitro
AU - Spanoudes, Kyriakos
AU - Gaspar, Diana
AU - Pandit, Abhay
AU - Zeugolis, Dimitrios I.
PY - 2014/9
Y1 - 2014/9
N2 - Tendon injuries constitute an unmet clinical need, with 3 to 5 million new incidents occurring annually worldwide. Tissue grafting and biomaterial-based approaches fail to provide environments that are conducive to regeneration; instead they lead to nonspecific cell adhesion and scar tissue formation, which collectively impair functionality. Cell based therapies may potentially recover native tendon function, if tenocyte trans-differentiation can be evaded and stem cell differentiation towards tenogenic lineage is attained. To this end, recreating an artificial in vivo tendon niche by engineering functional in vitro microenvironments is a research priority. Clinically relevant cell based therapies for tendon repair and regeneration could be created using tools that harness biophysical beacons (surface topography, mechanical loading), biochemical cues (oxygen tension), and biological signals (growth factors).
AB - Tendon injuries constitute an unmet clinical need, with 3 to 5 million new incidents occurring annually worldwide. Tissue grafting and biomaterial-based approaches fail to provide environments that are conducive to regeneration; instead they lead to nonspecific cell adhesion and scar tissue formation, which collectively impair functionality. Cell based therapies may potentially recover native tendon function, if tenocyte trans-differentiation can be evaded and stem cell differentiation towards tenogenic lineage is attained. To this end, recreating an artificial in vivo tendon niche by engineering functional in vitro microenvironments is a research priority. Clinically relevant cell based therapies for tendon repair and regeneration could be created using tools that harness biophysical beacons (surface topography, mechanical loading), biochemical cues (oxygen tension), and biological signals (growth factors).
KW - In vitro tools
KW - Tendon cell based therapies
KW - Tenogenic differentiation
KW - Tenogenic phenotype maintenance
UR - http://www.scopus.com/inward/record.url?scp=84906303707&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2014.06.009
DO - 10.1016/j.tibtech.2014.06.009
M3 - Review article
C2 - 25043371
AN - SCOPUS:84906303707
SN - 0167-7799
VL - 32
SP - 474
EP - 482
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 9
ER -