Systematic evaluation of distribution of transgene expression after adenovirus-mediated gene transfer to the transplanted heart

Carlo Pellegrini; Timothy O'Brien; John Yap; Anders Jeppsson; Henry D. Tazelaar; Christopher G.A. McGregor

doi:10.1007/s001470050160

Systematic evaluation of distribution of transgene expression after adenovirus-mediated gene transfer to the transplanted heart

Carlo Pellegrini, Timothy O'Brien, John Yap, Anders Jeppsson, Henry D. Tazelaar, Christopher G.A. McGregor

Mayo Clinic

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

11 Citations (Scopus)

Abstract

In the transplantation setting, the study and potential treatment of acute and chronic rejection by means of gene therapy will require widespread transgene expression in the donor organ. The distribution of transgene expression after adenovirus-mediated gene transfer via the coronary vasculature in a model of abdominal heterotopic heart transplantation in syngeneic rats (n = 6) was evaluated at 1 week. Reporter gene expression was evenly distributed in the base, the midventricle, and the apex of the transplanted hearts. This study demonstrates that intracoronary administration of the adenoviral vector to the donor heart results in widespread transgene expression.

Original language	English
Pages (from-to)	373-377
Number of pages	5
Journal	Transplant International
Volume	11
Issue number	5
DOIs	https://doi.org/10.1007/s001470050160
Publication status	Published - Sep 1998
Externally published	Yes

Keywords

Adenovirus, heart, transplantation
Gene therapy, heart transplantation
Gene transfer, heart transplantation
Heart transplantation, gene therapy

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10.1007/s001470050160

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title = "Systematic evaluation of distribution of transgene expression after adenovirus-mediated gene transfer to the transplanted heart",

abstract = "In the transplantation setting, the study and potential treatment of acute and chronic rejection by means of gene therapy will require widespread transgene expression in the donor organ. The distribution of transgene expression after adenovirus-mediated gene transfer via the coronary vasculature in a model of abdominal heterotopic heart transplantation in syngeneic rats (n = 6) was evaluated at 1 week. Reporter gene expression was evenly distributed in the base, the midventricle, and the apex of the transplanted hearts. This study demonstrates that intracoronary administration of the adenoviral vector to the donor heart results in widespread transgene expression.",

keywords = "Adenovirus, heart, transplantation, Gene therapy, heart transplantation, Gene transfer, heart transplantation, Heart transplantation, gene therapy",

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AU - Pellegrini, Carlo

AU - O'Brien, Timothy

AU - Yap, John

AU - Jeppsson, Anders

AU - Tazelaar, Henry D.

AU - McGregor, Christopher G.A.

PY - 1998/9

Y1 - 1998/9

N2 - In the transplantation setting, the study and potential treatment of acute and chronic rejection by means of gene therapy will require widespread transgene expression in the donor organ. The distribution of transgene expression after adenovirus-mediated gene transfer via the coronary vasculature in a model of abdominal heterotopic heart transplantation in syngeneic rats (n = 6) was evaluated at 1 week. Reporter gene expression was evenly distributed in the base, the midventricle, and the apex of the transplanted hearts. This study demonstrates that intracoronary administration of the adenoviral vector to the donor heart results in widespread transgene expression.

AB - In the transplantation setting, the study and potential treatment of acute and chronic rejection by means of gene therapy will require widespread transgene expression in the donor organ. The distribution of transgene expression after adenovirus-mediated gene transfer via the coronary vasculature in a model of abdominal heterotopic heart transplantation in syngeneic rats (n = 6) was evaluated at 1 week. Reporter gene expression was evenly distributed in the base, the midventricle, and the apex of the transplanted hearts. This study demonstrates that intracoronary administration of the adenoviral vector to the donor heart results in widespread transgene expression.

KW - Adenovirus, heart, transplantation

KW - Gene therapy, heart transplantation

KW - Gene transfer, heart transplantation

KW - Heart transplantation, gene therapy

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ER -

Systematic evaluation of distribution of transgene expression after adenovirus-mediated gene transfer to the transplanted heart

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Keywords

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