Supramolecular hydrogels with reverse thermal gelation properties from (Oligo)tyrosine containing block copolymers

Jin Huang; Conn L. Hastings; Garry P. Duffy; Helena M. Kelly; Jaclyn Raeburn; Dave J. Adams; Andreas Heise

doi:10.1021/bm301629f

Supramolecular hydrogels with reverse thermal gelation properties from (Oligo)tyrosine containing block copolymers

Jin Huang, Conn L. Hastings, Garry P. Duffy, Helena M. Kelly, Jaclyn Raeburn, Dave J. Adams, Andreas Heise

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

113 Citations (Scopus)

Abstract

Novel block copolymers comprising poly(ethylene glycol) (PEG) and an oligo(tyrosine) block were synthesized in different compositions by N-carboxyanhydride (NCA) polymerization. It was shown that PEG2000-Tyr ₆ undergoes thermoresponsive hydrogelation at a low concentration range of 0.25-3.0 wt % within a temperature range of 25-50 C. Cryogenic transmission electron microscopy (Cryo-TEM) revealed a continuous network of fibers throughout the hydrogel sample, even at concentrations as low as 0.25 wt %. Circular dichroism (CD) results suggest that better packing of the β-sheet tyrosine block at increasing temperature induces the reverse thermogelation. A preliminary assessment of the potential of the hydrogel for in vitro application confirmed the hydrogel is not cytotoxic, is biodegradable, and produced a sustained release of a small-molecule drug.

Original language	English
Pages (from-to)	200-206
Number of pages	7
Journal	Biomacromolecules
Volume	14
Issue number	1
DOIs	https://doi.org/10.1021/bm301629f
Publication status	Published - 14 Jan 2013
Externally published	Yes

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title = "Supramolecular hydrogels with reverse thermal gelation properties from (Oligo)tyrosine containing block copolymers",

abstract = "Novel block copolymers comprising poly(ethylene glycol) (PEG) and an oligo(tyrosine) block were synthesized in different compositions by N-carboxyanhydride (NCA) polymerization. It was shown that PEG2000-Tyr 6 undergoes thermoresponsive hydrogelation at a low concentration range of 0.25-3.0 wt \% within a temperature range of 25-50 C. Cryogenic transmission electron microscopy (Cryo-TEM) revealed a continuous network of fibers throughout the hydrogel sample, even at concentrations as low as 0.25 wt \%. Circular dichroism (CD) results suggest that better packing of the β-sheet tyrosine block at increasing temperature induces the reverse thermogelation. A preliminary assessment of the potential of the hydrogel for in vitro application confirmed the hydrogel is not cytotoxic, is biodegradable, and produced a sustained release of a small-molecule drug.",

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T1 - Supramolecular hydrogels with reverse thermal gelation properties from (Oligo)tyrosine containing block copolymers

AU - Huang, Jin

AU - Hastings, Conn L.

AU - Duffy, Garry P.

AU - Kelly, Helena M.

AU - Raeburn, Jaclyn

AU - Adams, Dave J.

AU - Heise, Andreas

PY - 2013/1/14

Y1 - 2013/1/14

N2 - Novel block copolymers comprising poly(ethylene glycol) (PEG) and an oligo(tyrosine) block were synthesized in different compositions by N-carboxyanhydride (NCA) polymerization. It was shown that PEG2000-Tyr 6 undergoes thermoresponsive hydrogelation at a low concentration range of 0.25-3.0 wt % within a temperature range of 25-50 C. Cryogenic transmission electron microscopy (Cryo-TEM) revealed a continuous network of fibers throughout the hydrogel sample, even at concentrations as low as 0.25 wt %. Circular dichroism (CD) results suggest that better packing of the β-sheet tyrosine block at increasing temperature induces the reverse thermogelation. A preliminary assessment of the potential of the hydrogel for in vitro application confirmed the hydrogel is not cytotoxic, is biodegradable, and produced a sustained release of a small-molecule drug.

AB - Novel block copolymers comprising poly(ethylene glycol) (PEG) and an oligo(tyrosine) block were synthesized in different compositions by N-carboxyanhydride (NCA) polymerization. It was shown that PEG2000-Tyr 6 undergoes thermoresponsive hydrogelation at a low concentration range of 0.25-3.0 wt % within a temperature range of 25-50 C. Cryogenic transmission electron microscopy (Cryo-TEM) revealed a continuous network of fibers throughout the hydrogel sample, even at concentrations as low as 0.25 wt %. Circular dichroism (CD) results suggest that better packing of the β-sheet tyrosine block at increasing temperature induces the reverse thermogelation. A preliminary assessment of the potential of the hydrogel for in vitro application confirmed the hydrogel is not cytotoxic, is biodegradable, and produced a sustained release of a small-molecule drug.

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U2 - 10.1021/bm301629f

DO - 10.1021/bm301629f

M3 - Article

SN - 1525-7797

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EP - 206

JO - Biomacromolecules

JF - Biomacromolecules

IS - 1

ER -

Supramolecular hydrogels with reverse thermal gelation properties from (Oligo)tyrosine containing block copolymers

Abstract

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