Structure of the O-specific polysaccharide from Shewanella japonica type strain KMM 3299T containing the rare amino sugar Fuc4NAc

Michelle Kilcoyne; Alexander S. Shashkov; Andrei V. Perepelov; Evgeny L. Nazarenko; Raisa P. Gorshkova; Elena P. Ivanova; Göran Widmalm; Angela V. Savage

doi:10.1016/j.carres.2005.01.040

Structure of the O-specific polysaccharide from Shewanella japonica type strain KMM 3299^T containing the rare amino sugar Fuc4NAc

Michelle Kilcoyne, Alexander S. Shashkov, Andrei V. Perepelov, Evgeny L. Nazarenko, Raisa P. Gorshkova, Elena P. Ivanova, Göran Widmalm, Angela V. Savage

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

9 Citations (Scopus)

Abstract

An acidic O-specific polysaccharide (PS) of the agar-digesting bacterium Shewanella japonica with the type strain KMM 3299^T was obtained by mild acid hydrolysis of the lipopolysaccharide. The polysaccharide was studied by component analysis, methylation analysis, ¹H and ¹³C NMR spectroscopy, including 2D NMR experiments. The PS was determined to have the following structure involving three unusual amino sugars:→3)-α-d- Fucp4NAc-(1→4)-α-d-GalpA-(1→3)-α-l-FucpNAc-(1→3) -β-d-QuipNAc4NAc-(1→

Original language	English
Pages (from-to)	1557-1561
Number of pages	5
Journal	Carbohydrate Research
Volume	340
Issue number	8
DOIs	https://doi.org/10.1016/j.carres.2005.01.040
Publication status	Published - 13 Jun 2005

Keywords

2,4-Diacetamido-2,4,6-trideoxy-d-glucose
2-Acetamido-2,6-dideoxy-l-galactose
4-Acetamido-4,6-dideoxy-d- galactose
Bacillosamine
Marine bacteria
NMR spectroscopy
Polysaccharides
Shewanella japonica
Structure

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10.1016/j.carres.2005.01.040

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@article{2e027e55342446a893b30896956e76d0,

title = "Structure of the O-specific polysaccharide from Shewanella japonica type strain KMM 3299T containing the rare amino sugar Fuc4NAc",

abstract = "An acidic O-specific polysaccharide (PS) of the agar-digesting bacterium Shewanella japonica with the type strain KMM 3299T was obtained by mild acid hydrolysis of the lipopolysaccharide. The polysaccharide was studied by component analysis, methylation analysis, 1H and 13C NMR spectroscopy, including 2D NMR experiments. The PS was determined to have the following structure involving three unusual amino sugars:→3)-α-d- Fucp4NAc-(1→4)-α-d-GalpA-(1→3)-α-l-FucpNAc-(1→3) -β-d-QuipNAc4NAc-(1→",

keywords = "2,4-Diacetamido-2,4,6-trideoxy-d-glucose, 2-Acetamido-2,6-dideoxy-l-galactose, 4-Acetamido-4,6-dideoxy-d- galactose, Bacillosamine, Marine bacteria, NMR spectroscopy, Polysaccharides, Shewanella japonica, Structure",

author = "Michelle Kilcoyne and Shashkov, \{Alexander S.\} and Perepelov, \{Andrei V.\} and Nazarenko, \{Evgeny L.\} and Gorshkova, \{Raisa P.\} and Ivanova, \{Elena P.\} and G{\"o}ran Widmalm and Savage, \{Angela V.\}",

year = "2005",

month = jun,

day = "13",

doi = "10.1016/j.carres.2005.01.040",

language = "English",

volume = "340",

pages = "1557--1561",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier Ltd",

number = "8",

}

TY - JOUR

T1 - Structure of the O-specific polysaccharide from Shewanella japonica type strain KMM 3299T containing the rare amino sugar Fuc4NAc

AU - Kilcoyne, Michelle

AU - Shashkov, Alexander S.

AU - Perepelov, Andrei V.

AU - Nazarenko, Evgeny L.

AU - Gorshkova, Raisa P.

AU - Ivanova, Elena P.

AU - Widmalm, Göran

AU - Savage, Angela V.

PY - 2005/6/13

Y1 - 2005/6/13

N2 - An acidic O-specific polysaccharide (PS) of the agar-digesting bacterium Shewanella japonica with the type strain KMM 3299T was obtained by mild acid hydrolysis of the lipopolysaccharide. The polysaccharide was studied by component analysis, methylation analysis, 1H and 13C NMR spectroscopy, including 2D NMR experiments. The PS was determined to have the following structure involving three unusual amino sugars:→3)-α-d- Fucp4NAc-(1→4)-α-d-GalpA-(1→3)-α-l-FucpNAc-(1→3) -β-d-QuipNAc4NAc-(1→

AB - An acidic O-specific polysaccharide (PS) of the agar-digesting bacterium Shewanella japonica with the type strain KMM 3299T was obtained by mild acid hydrolysis of the lipopolysaccharide. The polysaccharide was studied by component analysis, methylation analysis, 1H and 13C NMR spectroscopy, including 2D NMR experiments. The PS was determined to have the following structure involving three unusual amino sugars:→3)-α-d- Fucp4NAc-(1→4)-α-d-GalpA-(1→3)-α-l-FucpNAc-(1→3) -β-d-QuipNAc4NAc-(1→

KW - 2,4-Diacetamido-2,4,6-trideoxy-d-glucose

KW - 2-Acetamido-2,6-dideoxy-l-galactose

KW - 4-Acetamido-4,6-dideoxy-d- galactose

KW - Bacillosamine

KW - Marine bacteria

KW - NMR spectroscopy

KW - Polysaccharides

KW - Shewanella japonica

KW - Structure

UR - https://www.scopus.com/pages/publications/19344367686

U2 - 10.1016/j.carres.2005.01.040

DO - 10.1016/j.carres.2005.01.040

M3 - Article

SN - 0008-6215

VL - 340

SP - 1557

EP - 1561

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 8

ER -

Structure of the O-specific polysaccharide from Shewanella japonica type strain KMM 3299^T containing the rare amino sugar Fuc4NAc

Abstract

Keywords

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