Staphylococcus aureus Colony Polymerase Chain Reaction

Merve S. Zeden; Christopher F. Schuster; Angelika Gründling

doi:10.1101/pdb.prot107949

Staphylococcus aureus Colony Polymerase Chain Reaction

Merve S. Zeden
, Christopher F. Schuster
, Angelika Gründling

Infectious Disease

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

8 Citations (Scopus)

Abstract

Here, we describe a protocol for a colony polymerase chain reaction (PCR) method for Staphylococcus aureus. The methodology involves the preparation of small S. aureus lysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.

Original language	English
Pages (from-to)	562-566
Number of pages	5
Journal	Cold Spring Harbor Protocols
Volume	2023
Issue number	8
DOIs	https://doi.org/10.1101/pdb.prot107949
Publication status	Published - 2023

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10.1101/pdb.prot107949

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title = "Staphylococcus aureus Colony Polymerase Chain Reaction",

abstract = "Here, we describe a protocol for a colony polymerase chain reaction (PCR) method for Staphylococcus aureus. The methodology involves the preparation of small S. aureus lysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.",

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AU - Schuster, Christopher F.

AU - Gründling, Angelika

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AB - Here, we describe a protocol for a colony polymerase chain reaction (PCR) method for Staphylococcus aureus. The methodology involves the preparation of small S. aureus lysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.

UR - https://www.scopus.com/pages/publications/85166385215

U2 - 10.1101/pdb.prot107949

DO - 10.1101/pdb.prot107949

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SP - 562

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JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

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Staphylococcus aureus Colony Polymerase Chain Reaction

Abstract

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