Spectrophotometric Microdetermination of Amines and Sulfamates with 1,4-Benzoquinone

Twenty aliphatic, primary and secondary, alicyclic and heterocyclic amines In chloroform have been determined spectrophotometrically by measurement of the absorbance (λ_max 478 to 510 nm) of the reaction product from their reaction at 60 °C with 1% ethanolic 1,4-benzoquinone. Cyclopentyl- and cycloheptylamines and the artificial sweeteners cyclopentyl-, cycloheptyl-, and cyclooctylsulfamates have been determined after extraction from urine. The mean per cent recoveries ± average deviation for cyclopentylamine and cycloheptylsulfamate In urine are 97 ± 1.99 and 103.61 ± 4.42, respectively. Beer's law was followed In all cases over an approximate range of 5-100 μg/ml. The sensitivity of the method (using cyclopentylamine) was 3 μg/ml (2 ppm). A precision study showed that the relative standard deviation of absorbance readings was 0.6% for n-octylamine.