Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram; Jared Q. Gerlach; Yun Xiang; Abdel Nasser Kawde; Zong Dai; Veer P. Bhavanandan; Jeffrey T. La Belle; Waret Veerasai; Lokesh Joshi; Joseph Wang

doi:10.1039/b605943k

Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram
, Jared Q. Gerlach
, Yun Xiang
, Abdel Nasser Kawde
, Zong Dai
, Veer P. Bhavanandan
, Jeffrey T. La Belle
, Waret Veerasai
, Lokesh Joshi
, Joseph Wang

Infectious Disease

Arizona State University
Faculty of Science, Mahidol University

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

3 Citations (Scopus)

Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

Original language	English
Pages (from-to)	889-891
Number of pages	3
Journal	Analyst
Volume	131
Issue number	8
DOIs	https://doi.org/10.1039/b605943k
Publication status	Published - 1 Aug 2006

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10.1039/b605943k

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abstract = "Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.",

author = "Tanin Tangkuaram and Gerlach, \{Jared Q.\} and Yun Xiang and Kawde, \{Abdel Nasser\} and Zong Dai and Bhavanandan, \{Veer P.\} and \{La Belle\}, \{Jeffrey T.\} and Waret Veerasai and Lokesh Joshi and Joseph Wang",

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T1 - Sensitive and rapid electrochemical bioassay of glycosidase activity

AU - Tangkuaram, Tanin

AU - Gerlach, Jared Q.

AU - Xiang, Yun

AU - Kawde, Abdel Nasser

AU - Dai, Zong

AU - Bhavanandan, Veer P.

AU - La Belle, Jeffrey T.

AU - Veerasai, Waret

AU - Joshi, Lokesh

AU - Wang, Joseph

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

AB - Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

UR - https://www.scopus.com/pages/publications/33746437088

UR - http://europepmc.org/abstract/med/17028720

U2 - 10.1039/b605943k

DO - 10.1039/b605943k

M3 - Article

C2 - 17028720

SN - 0003-2654

VL - 131

SP - 889

EP - 891

JO - Analyst

JF - Analyst

IS - 8

ER -

Sensitive and rapid electrochemical bioassay of glycosidase activity

Abstract

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