Abstract
In this work we report for the first time on a
repertoire of valving technologies which are combined to enable automated
purification of total RNA from cell
homogenate. Process control is implemented using rotational-pulse actuated dissolvable-film
(DF) valves; where the order of valve actuation is determined by the disc
architecture while the timing of valve actuation is governed by pulses in the spin
rate. Selective liquid routing is enabled by combining a heavy, inert and
immiscible liquid plug with a DF. Combined, these technologies enable
bead-based extraction of amplifiable RNA, with a yield comparable to
gold-standard bench-top protocols.
| Original language | English (Ireland) |
|---|---|
| Title of host publication | MicroTAS 2014 |
| Publication status | Published - 1 Oct 2014 |
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Kinahan, D J., Clancy, E., Kilcawley, N.A., Dimov, N., Smith T. J. and Ducrée J.