@inproceedings{865b8df414864e2195c1c020f7580028,
title = "Rotational-pulse actuated dissolvable-film valves for automated purification of total RNA from E. Coli",
abstract = "In this work we report for the first time on a repertoire of valving technologies which are combined to enable automated purification of total RNA from cell homogenate. Process control is implemented using rotational-pulse actuated dissolvable-film (DF) valves; where the order of valve actuation is determined by the disc architecture while the timing of valve actuation is governed by pulses in the spin rate. Selective liquid routing is enabled by combining a heavy, inert and immiscible liquid plug with a DF. The combination of these technologies enables bead-based extraction of amplifiable RNA, with a yield comparable to gold-standard bench-top protocols.",
keywords = "Dissolvable films, Lab-on-a-Disc, Nucleic acid, Sample preparation, Valving",
author = "Kinahan, \{D. J.\} and E. Clancy and Kilcawley, \{N. A.\} and N. Dimov and Smith, \{T. J.\} and J. Ducr{\'e}e",
note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",
year = "2014",
language = "English",
series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",
pages = "1437--1439",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
}