Rooting Species Trees Using Gene Tree-Species Tree Reconciliation

Brogan J. Harris, Paul O. Sheridan, Adrián A. Davín, Cécile Gubry-Rangin, Gergely J. Szöllősi, Tom A. Williams

Research output: Chapter in Book or Conference Publication/ProceedingChapterpeer-review

2 Citations (Scopus)

Abstract

Interpreting phylogenetic trees requires a root, which provides the direction of evolution and polarizes ancestor-descendant relationships. But inferring the root using genetic data is difficult, particularly in cases where the closest available outgroup is only distantly related, which are common for microbes. In this chapter, we present a workflow for estimating rooted species trees and the evolutionary history of the gene families that evolve within them using probabilistic gene tree-species tree reconciliation. We illustrate the pipeline using a small dataset of prokaryotic genomes, for which the example scripts can be run using modest computer resources. We describe the rooting method used in this work in the context or other rooting strategies and discuss some of the limitations and opportunities presented by probabilistic gene tree-species tree reconciliation methods.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages189-211
Number of pages23
DOIs
Publication statusPublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2569
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Amalgamated likelihood estimation
  • Evolution
  • Phylogenetics
  • Reconciliation
  • Rooting

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