Abstract
A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA before being subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10(12) cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.
| Original language | English (Ireland) |
|---|---|
| Number of pages | 11 |
| Journal | Journal Of Rapid Methods & Automation In Microbiology |
| Volume | 16 |
| Publication status | Published - 1 Sep 2008 |
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Glynn, B;Lacey, K;O'Grady, J;Barry, T;Smith, TJ;Maher, M
