TY - JOUR
T1 - Resolution of human ribosomal dna occurs in anaphase, dependent on tankyrase 1, condensin II, and topoisomerase IIα
AU - Daniloski, Zharko
AU - Bisht, Kamlesh K.
AU - McStay, Brian
AU - Smith, Susan
N1 - Publisher Copyright:
© 2019 Daniloski et al.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Formation of individualized sister chromatids is essential for their accurate segregation. In budding yeast, while most of the genome segregates at the metaphase to anaphase transition, resolution of the ribosomal DNA (rDNA) repeats is delayed. The timing and mechanism in human cells is unknown. Here we show that resolution of human rDNA occurs in anaphase after the bulk of the genome, dependent on tankyrase 1, condensin II, and topoisomerase IIα. Defective resolution leads to rDNA bridges, rDNA damage, and aneuploidy of an rDNA-con-taining acrocentric chromosome. Thus, temporal regulation of rDNA segregation is conserved between yeast and man and is essential for genome integrity.
AB - Formation of individualized sister chromatids is essential for their accurate segregation. In budding yeast, while most of the genome segregates at the metaphase to anaphase transition, resolution of the ribosomal DNA (rDNA) repeats is delayed. The timing and mechanism in human cells is unknown. Here we show that resolution of human rDNA occurs in anaphase after the bulk of the genome, dependent on tankyrase 1, condensin II, and topoisomerase IIα. Defective resolution leads to rDNA bridges, rDNA damage, and aneuploidy of an rDNA-con-taining acrocentric chromosome. Thus, temporal regulation of rDNA segregation is conserved between yeast and man and is essential for genome integrity.
KW - Condensin
KW - RDNA
KW - Tankyrase
KW - Topoisomerase IIa
UR - http://www.scopus.com/inward/record.url?scp=85063294596&partnerID=8YFLogxK
U2 - 10.1101/gad.321836.118
DO - 10.1101/gad.321836.118
M3 - Article
SN - 0890-9369
VL - 33
SP - 276
EP - 281
JO - Genes and Development
JF - Genes and Development
IS - 5-6
ER -