Reevaluation of contemporary laboratory methods for detection of antimicrobial resistance among enterococci

Martin G. Cormican; Douglas Biedenbach; Ronald N. Jones

doi:10.1111/j.1469-0691.1996.tb00552.x

Reevaluation of contemporary laboratory methods for detection of antimicrobial resistance among enterococci

Martin G. Cormican, Douglas Biedenbach, Ronald N. Jones

University of Iowa Carver College of Medicine

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

4 Citations (Scopus)

Abstract

Objective: To evaluate broth microdilution, disk diffusion, Etest and Vitek Systems for susceptibility testing of enterococci. Methods: Susceptibility testing of a panel of 149 enterococci (99 vancomycin-resistant) strains, using the study methods, was performed and the results compared. Results: For vancomycin susceptibility testing, categorical agreement of disk diffusion, Etest and Vitek with the reference broth microdilution test was > 95%. For aminoglycoside and ampicillin testing, categorical agreement between Etest and Vitek was 98 to 100%. Conclusions: Disk diffusion, Etest and Vitek have acceptable performance for detection of vancomycin resistance of Van A and Van B phenotypes among enterococci.

Original language	English
Pages (from-to)	190-194
Number of pages	5
Journal	Clinical Microbiology and Infection
Volume	1
Issue number	3
DOIs	https://doi.org/10.1111/j.1469-0691.1996.tb00552.x
Publication status	Published - Mar 1996
Externally published	Yes

Keywords

Enterococcus faecium
Resistant enterococci
Susceptibility testing
Vancomycin
Vitek

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10.1111/j.1469-0691.1996.tb00552.x

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title = "Reevaluation of contemporary laboratory methods for detection of antimicrobial resistance among enterococci",

abstract = "Objective: To evaluate broth microdilution, disk diffusion, Etest and Vitek Systems for susceptibility testing of enterococci. Methods: Susceptibility testing of a panel of 149 enterococci (99 vancomycin-resistant) strains, using the study methods, was performed and the results compared. Results: For vancomycin susceptibility testing, categorical agreement of disk diffusion, Etest and Vitek with the reference broth microdilution test was > 95\%. For aminoglycoside and ampicillin testing, categorical agreement between Etest and Vitek was 98 to 100\%. Conclusions: Disk diffusion, Etest and Vitek have acceptable performance for detection of vancomycin resistance of Van A and Van B phenotypes among enterococci.",

keywords = "Enterococcus faecium, Resistant enterococci, Susceptibility testing, Vancomycin, Vitek",

author = "Cormican, \{Martin G.\} and Douglas Biedenbach and Jones, \{Ronald N.\}",

year = "1996",

month = mar,

doi = "10.1111/j.1469-0691.1996.tb00552.x",

language = "English",

volume = "1",

pages = "190--194",

journal = "Clinical Microbiology and Infection",

issn = "1198-743X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Reevaluation of contemporary laboratory methods for detection of antimicrobial resistance among enterococci

AU - Cormican, Martin G.

AU - Biedenbach, Douglas

AU - Jones, Ronald N.

PY - 1996/3

Y1 - 1996/3

N2 - Objective: To evaluate broth microdilution, disk diffusion, Etest and Vitek Systems for susceptibility testing of enterococci. Methods: Susceptibility testing of a panel of 149 enterococci (99 vancomycin-resistant) strains, using the study methods, was performed and the results compared. Results: For vancomycin susceptibility testing, categorical agreement of disk diffusion, Etest and Vitek with the reference broth microdilution test was > 95%. For aminoglycoside and ampicillin testing, categorical agreement between Etest and Vitek was 98 to 100%. Conclusions: Disk diffusion, Etest and Vitek have acceptable performance for detection of vancomycin resistance of Van A and Van B phenotypes among enterococci.

AB - Objective: To evaluate broth microdilution, disk diffusion, Etest and Vitek Systems for susceptibility testing of enterococci. Methods: Susceptibility testing of a panel of 149 enterococci (99 vancomycin-resistant) strains, using the study methods, was performed and the results compared. Results: For vancomycin susceptibility testing, categorical agreement of disk diffusion, Etest and Vitek with the reference broth microdilution test was > 95%. For aminoglycoside and ampicillin testing, categorical agreement between Etest and Vitek was 98 to 100%. Conclusions: Disk diffusion, Etest and Vitek have acceptable performance for detection of vancomycin resistance of Van A and Van B phenotypes among enterococci.

KW - Enterococcus faecium

KW - Resistant enterococci

KW - Susceptibility testing

KW - Vancomycin

KW - Vitek

UR - https://www.scopus.com/pages/publications/0009539279

U2 - 10.1111/j.1469-0691.1996.tb00552.x

DO - 10.1111/j.1469-0691.1996.tb00552.x

M3 - Article

AN - SCOPUS:0009539279

SN - 1198-743X

VL - 1

SP - 190

EP - 194

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 3

ER -

Reevaluation of contemporary laboratory methods for detection of antimicrobial resistance among enterococci

Abstract

Keywords

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