RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene

Shirong Yu; Yumin Teng; Noel F. Lowndes; Raymond Waters

doi:10.1016/S0921-8777(01)00061-1

RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene

Shirong Yu
, Yumin Teng
, Noel F. Lowndes
, Raymond Waters

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

17 Citations (Scopus)

Abstract

In this study, the effect of a prior UV irradiation on the removal of cyclobutane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-transcribed strand (NTS) of the MFA2 gene in haploid Saccharomyces cerevisiae (S. cerevisiae) cells was investigated. In NER competent cells, the pre-irradiation with a dose of 20 J/m² enhances the removal of CPDs induced by a second UV dose of 100 J/m² in the TS and the NTS of MFA2 gene except for the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the transcription coupled repair gene RAD26 are essential for inducible NER.

Original language	English
Pages (from-to)	229-236
Number of pages	8
Journal	Mutation Research-DNA Repair
Volume	485
Issue number	3
DOIs	https://doi.org/10.1016/S0921-8777(01)00061-1
Publication status	Published - 4 Apr 2001
Externally published	Yes

Keywords

Cyclobutane pyrimidine dimers
Inducible repair
MFA2
Nucleotide excision repair
Sacchromyces cerevisiae

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10.1016/S0921-8777(01)00061-1

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Yu, S., Teng, Y., Lowndes, N. F., & Waters, R. (2001). RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene. Mutation Research-DNA Repair, 485(3), 229-236. https://doi.org/10.1016/S0921-8777(01)00061-1

@article{4c90098587df41db8d3edd5d0f7c2762,

title = "RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene",

abstract = "In this study, the effect of a prior UV irradiation on the removal of cyclobutane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-transcribed strand (NTS) of the MFA2 gene in haploid Saccharomyces cerevisiae (S. cerevisiae) cells was investigated. In NER competent cells, the pre-irradiation with a dose of 20 J/m2 enhances the removal of CPDs induced by a second UV dose of 100 J/m2 in the TS and the NTS of MFA2 gene except for the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the transcription coupled repair gene RAD26 are essential for inducible NER.",

keywords = "Cyclobutane pyrimidine dimers, Inducible repair, MFA2, Nucleotide excision repair, Sacchromyces cerevisiae",

author = "Shirong Yu and Yumin Teng and Lowndes, \{Noel F.\} and Raymond Waters",

year = "2001",

month = apr,

day = "4",

doi = "10.1016/S0921-8777(01)00061-1",

language = "English",

volume = "485",

pages = "229--236",

journal = "Mutation Research-DNA Repair",

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publisher = "Elsevier B.V.",

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Yu, S, Teng, Y, Lowndes, NF & Waters, R 2001, 'RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene', Mutation Research-DNA Repair, vol. 485, no. 3, pp. 229-236. https://doi.org/10.1016/S0921-8777(01)00061-1

RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene. / Yu, Shirong; Teng, Yumin; Lowndes, Noel F. et al.
In: Mutation Research-DNA Repair, Vol. 485, No. 3, 04.04.2001, p. 229-236.

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

TY - JOUR

T1 - RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene

AU - Yu, Shirong

AU - Teng, Yumin

AU - Lowndes, Noel F.

AU - Waters, Raymond

PY - 2001/4/4

Y1 - 2001/4/4

N2 - In this study, the effect of a prior UV irradiation on the removal of cyclobutane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-transcribed strand (NTS) of the MFA2 gene in haploid Saccharomyces cerevisiae (S. cerevisiae) cells was investigated. In NER competent cells, the pre-irradiation with a dose of 20 J/m2 enhances the removal of CPDs induced by a second UV dose of 100 J/m2 in the TS and the NTS of MFA2 gene except for the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the transcription coupled repair gene RAD26 are essential for inducible NER.

AB - In this study, the effect of a prior UV irradiation on the removal of cyclobutane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-transcribed strand (NTS) of the MFA2 gene in haploid Saccharomyces cerevisiae (S. cerevisiae) cells was investigated. In NER competent cells, the pre-irradiation with a dose of 20 J/m2 enhances the removal of CPDs induced by a second UV dose of 100 J/m2 in the TS and the NTS of MFA2 gene except for the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the transcription coupled repair gene RAD26 are essential for inducible NER.

KW - Cyclobutane pyrimidine dimers

KW - Inducible repair

KW - MFA2

KW - Nucleotide excision repair

KW - Sacchromyces cerevisiae

UR - https://www.scopus.com/pages/publications/0035804789

U2 - 10.1016/S0921-8777(01)00061-1

DO - 10.1016/S0921-8777(01)00061-1

M3 - Article

SN - 0921-8777

VL - 485

SP - 229

EP - 236

JO - Mutation Research-DNA Repair

JF - Mutation Research-DNA Repair

IS - 3

ER -

RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene

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Keywords

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