Abstract
In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent(TRI) to pro-vide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase sepa-rated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent re-sistant cyclo olefin polymer (Zeonor) disc was used in this case.
| Original language | English (Ireland) |
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| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Place of Publication | Seattle, Washington, USA |
| Publication status | Published - 1 Oct 2011 |
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Linares, A.V., Gorkin, R., Glynn, B., Godino, N., Miller, N., Kerin, M., Barry, T., Smith, T. and Ducrée, J.