Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer

Terry Smith

Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer

Terry Smith

Infectious Disease

Research output: Chapter in Book or Conference Publication/Proceeding › Conference Publication › peer-review

5 Citations (Scopus)

Abstract

In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent(TRI) to pro-vide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase sepa-rated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent re-sistant cyclo olefin polymer (Zeonor) disc was used in this case.

Original language	English (Ireland)
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of Publication	Seattle, Washington, USA
Pages	1460-1462
Number of pages	3
Publication status	Published - 1 Oct 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 2 Oct 2011 → 6 Oct 2011

Publication series

Name	3

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/Territory	United States
City	Seattle, WA
Period	2/10/11 → 6/10/11

Keywords

Centrifugal microfluidics
MiRNA extraction
Microhomogenizer
Whole blood

Authors (Note for portal: view the doc link for the full list of authors)

Authors
Ana V. Linares, Robert Gorkin III, Barry Glynn, Neus Godino, Nicola Miller, Michael Kerin,Thomas Barry, Terry Smith, and Jens Ducrée

Cite this

@inproceedings{d48cefa7fead40eaaf289709808c9b56,

title = "Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer",

abstract = " In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent(TRI) to pro-vide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase sepa-rated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent re-sistant cyclo olefin polymer (Zeonor) disc was used in this case. ",

keywords = "Centrifugal microfluidics, MiRNA extraction, Microhomogenizer, Whole blood",

author = "Terry Smith",

year = "2011",

month = oct,

day = "1",

language = "English (Ireland)",

isbn = "9781618395955",

series = "3",

pages = "1460--1462",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

}

Smith, T 2011, Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences . 3, Seattle, Washington, USA, pp. 1460-1462, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 2/10/11.

Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer. / Smith, Terry.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences . Seattle, Washington, USA, 2011. p. 1460-1462 (3).

Research output: Chapter in Book or Conference Publication/Proceeding › Conference Publication › peer-review

TY - GEN

T1 - Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer

AU - Smith, Terry

PY - 2011/10/1

Y1 - 2011/10/1

N2 - In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent(TRI) to pro-vide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase sepa-rated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent re-sistant cyclo olefin polymer (Zeonor) disc was used in this case.

AB - In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent(TRI) to pro-vide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase sepa-rated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent re-sistant cyclo olefin polymer (Zeonor) disc was used in this case.

KW - Centrifugal microfluidics

KW - MiRNA extraction

KW - Microhomogenizer

KW - Whole blood

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M3 - Conference Publication

SN - 9781618395955

T3 - 3

SP - 1460

EP - 1462

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences

CY - Seattle, Washington, USA

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer

Abstract

Publication series

Conference

Keywords

Authors (Note for portal: view the doc link for the full list of authors)

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Cite this