TY - GEN
T1 - Post-self-assembly experimentation on extruded collagen fibres for tissue engineering applications
AU - Zeugolis, Dimitrios
PY - 2008/11/1
Y1 - 2008/11/1
N2 - Extruded collagen fibres have been shown to constitute a biomimetic three-dimensional scaffold with numerous tissue engineering applications. The multi-step fabrication process of this material provides opportunities for further advancements to improve the properties of the final product. Herein we investigated the influence of the post-self-assembly washing baths oil the structural, mechanical and thermal properties of these fibres. The surface morphology and the inter-fibre packing were similar for every treatment. The overnight incubation in isopropanol yielded fibres with the highest temperature and energy of denaturation (p 0.013). Typical s- and j-shape stress-strain curves were obtained for all treatments in the dry and wet state respectively. Rehydration of the fibres resulted in increased fibre diameter (P 0.006) and reduced stress (p 0.001), force (p 0.001) and modulus (P 0.002) values for every treatment. In the dry state, the alcohol-treated fibres were characterized by the highest stress (P 0.002) values; whilst in the wet state the Tris-HCI-treated fibres were the weakest (p 0.006). For every treatment, in both dry and wet state, a strong and inverse relationship between the fibre diameter and the stress at break was observed. Overall, the fibres produced were characterized by properties similar to those of native tissues. (C) 2003 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
AB - Extruded collagen fibres have been shown to constitute a biomimetic three-dimensional scaffold with numerous tissue engineering applications. The multi-step fabrication process of this material provides opportunities for further advancements to improve the properties of the final product. Herein we investigated the influence of the post-self-assembly washing baths oil the structural, mechanical and thermal properties of these fibres. The surface morphology and the inter-fibre packing were similar for every treatment. The overnight incubation in isopropanol yielded fibres with the highest temperature and energy of denaturation (p 0.013). Typical s- and j-shape stress-strain curves were obtained for all treatments in the dry and wet state respectively. Rehydration of the fibres resulted in increased fibre diameter (P 0.006) and reduced stress (p 0.001), force (p 0.001) and modulus (P 0.002) values for every treatment. In the dry state, the alcohol-treated fibres were characterized by the highest stress (P 0.002) values; whilst in the wet state the Tris-HCI-treated fibres were the weakest (p 0.006). For every treatment, in both dry and wet state, a strong and inverse relationship between the fibre diameter and the stress at break was observed. Overall, the fibres produced were characterized by properties similar to those of native tissues. (C) 2003 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
U2 - 10.1016/j.actbio.2008.05.015
DO - 10.1016/j.actbio.2008.05.015
M3 - Other contribution
VL - 4
ER -