TY - JOUR
T1 - Porcine submaxillary mucin contains α2→3‐ and α2→6‐linked N‐acetyl‐ and N‐glycolyl‐neuraminic acid
AU - SAVAGE, Angela V.
AU - KOPPEN, Piet L.
AU - SCHIPHORST, Wietske E.C.M.
AU - TRIPPELVITZ, Leo A.W.
AU - VAN HALBEEK, Herman
AU - VLIEGENTHART, Johannes F.G.
AU - VAN DEN EIJNDEN, Dirk H.
PY - 1986/10
Y1 - 1986/10
N2 - Four acidic trisaccharides have been obtained by alkaline borohydride reductive cleavage (β‐elimination) of a fraction of porcine submaxillary mucin precipitating at 60 – 75% ethanol. Their structures have been investigated using the techniques of methylation analysis involving gas‐liquid chromatography/mass spectrometry along with high‐resolution 1H‐NMR analysis. Two of the four oligosaccharides, B1 – B4, contain N‐acetylneuraminic acid (NeuAc) while two contain N‐glycolylneuraminic acid (NeuGc). The following structures are proposed for the acidic trisaccharide fraction: (B1) NeuAcα(2→3)Galβ(1→3)GalNAcol, (B2) NeuGcα(2→3)Galβ(1→3)GalNAcol, (B3)Galβ(1→3)[NeuAcα(2→6)]GalNAcol and (B4) Galβ(1→3)[NeuGcα(2→6)]GalNAcol, (GalNAcol = reduced N‐acetylgalactosamine. These oligosaccharides were present in a molar ratio of 69:22:4:5. Although oligosaccharide B4 has previously been found in porcine submaxillary mucin B1, B2 and B3 have not. Furthermore, oligosaccharide B2 is a novel structure.
AB - Four acidic trisaccharides have been obtained by alkaline borohydride reductive cleavage (β‐elimination) of a fraction of porcine submaxillary mucin precipitating at 60 – 75% ethanol. Their structures have been investigated using the techniques of methylation analysis involving gas‐liquid chromatography/mass spectrometry along with high‐resolution 1H‐NMR analysis. Two of the four oligosaccharides, B1 – B4, contain N‐acetylneuraminic acid (NeuAc) while two contain N‐glycolylneuraminic acid (NeuGc). The following structures are proposed for the acidic trisaccharide fraction: (B1) NeuAcα(2→3)Galβ(1→3)GalNAcol, (B2) NeuGcα(2→3)Galβ(1→3)GalNAcol, (B3)Galβ(1→3)[NeuAcα(2→6)]GalNAcol and (B4) Galβ(1→3)[NeuGcα(2→6)]GalNAcol, (GalNAcol = reduced N‐acetylgalactosamine. These oligosaccharides were present in a molar ratio of 69:22:4:5. Although oligosaccharide B4 has previously been found in porcine submaxillary mucin B1, B2 and B3 have not. Furthermore, oligosaccharide B2 is a novel structure.
UR - http://www.scopus.com/inward/record.url?scp=0023017568&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1986.tb09948.x
DO - 10.1111/j.1432-1033.1986.tb09948.x
M3 - Article
C2 - 3769917
AN - SCOPUS:0023017568
SN - 0014-2956
VL - 160
SP - 123
EP - 129
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -