Optical fibre biosensors for oxygen and glucose monitoring

J. S. Young; P. J. Scully; F. Kvasnik; K. Rose; G. Kuncova; O. Podrazky; V. Matejec; J. Mrazek

doi:10.1117/12.623688

Optical fibre biosensors for oxygen and glucose monitoring

J. S. Young
, P. J. Scully
, F. Kvasnik
, K. Rose
, G. Kuncova
, O. Podrazky
, V. Matejec
, J. Mrazek

University of Manchester
Laser Technology North West Ltd.
Fraunhofer Institut Silicatforschung
Institute of Chemical Process Fundamentals of the CAS
Academy of Sciences of the Czech Republic

Research output: Contribution to a Journal (Peer & Non Peer) › Conference article › peer-review

5 Citations (Scopus)

Abstract

An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER®) is described. The Ruthenium/ ORMOCER® layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O₂)/litre, and the sensitivity is 70 ns/mg per litre.

Original language	English
Article number	104
Pages (from-to)	431-434
Number of pages	4
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5855 PART I
DOIs	https://doi.org/10.1117/12.623688
Publication status	Published - 2005
Externally published	Yes
Event	17th International Conference on Optical Fibre Sensors, OFS-17 - Bruges, Belgium Duration: 23 May 2005 → 27 May 2005

Keywords

Fluorescence
Glucose
Lifetime
Optical fibre biosensor
ORMOCER®
Oxygen
POF
Ruthenium

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10.1117/12.623688

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title = "Optical fibre biosensors for oxygen and glucose monitoring",

abstract = "An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER{\textregistered}) is described. The Ruthenium/ ORMOCER{\textregistered} layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.",

keywords = "Fluorescence, Glucose, Lifetime, Optical fibre biosensor, ORMOCER{\textregistered}, Oxygen, POF, Ruthenium",

author = "Young, \{J. S.\} and Scully, \{P. J.\} and F. Kvasnik and K. Rose and G. Kuncova and O. Podrazky and V. Matejec and J. Mrazek",

year = "2005",

doi = "10.1117/12.623688",

language = "English",

volume = "5855 PART I",

pages = "431--434",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "17th International Conference on Optical Fibre Sensors, OFS-17 ; Conference date: 23-05-2005 Through 27-05-2005",

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TY - JOUR

T1 - Optical fibre biosensors for oxygen and glucose monitoring

AU - Young, J. S.

AU - Scully, P. J.

AU - Kvasnik, F.

AU - Rose, K.

AU - Kuncova, G.

AU - Podrazky, O.

AU - Matejec, V.

AU - Mrazek, J.

PY - 2005

Y1 - 2005

N2 - An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER®) is described. The Ruthenium/ ORMOCER® layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.

AB - An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-ruthenium(II) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER®) is described. The Ruthenium/ ORMOCER® layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O2)/litre, and the sensitivity is 70 ns/mg per litre.

KW - Fluorescence

KW - Glucose

KW - Lifetime

KW - Optical fibre biosensor

KW - ORMOCER®

KW - Oxygen

KW - POF

KW - Ruthenium

UR - https://www.scopus.com/pages/publications/28544438697

U2 - 10.1117/12.623688

DO - 10.1117/12.623688

M3 - Conference article

AN - SCOPUS:28544438697

SN - 0277-786X

VL - 5855 PART I

SP - 431

EP - 434

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

M1 - 104

T2 - 17th International Conference on Optical Fibre Sensors, OFS-17

Y2 - 23 May 2005 through 27 May 2005

ER -

Optical fibre biosensors for oxygen and glucose monitoring

Abstract

Keywords

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