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Novel contrast mechanism for label free super-resolution imaging

  • Sergey A. Alexandrov
  • , James McGrath
  • , Hrebesh Subhash
  • , Francesca Boccafoschi
  • , Cinzia Giannini
  • , Martin Leahy
  • University of Galway
  • Dipartimento di Scienze della Salute
  • Consiglio Nazionale delle Ricerche

Research output: Chapter in Book or Conference Publication/ProceedingConference Publicationpeer-review

Abstract

We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form super-resolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXIII
EditorsCarol J. Cogswell, Tony Wilson, Thomas G. Brown
PublisherSPIE
ISBN (Electronic)9781628419474
DOIs
Publication statusPublished - 2016
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII - San Francisco, United States
Duration: 15 Feb 201617 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9713
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Country/TerritoryUnited States
CitySan Francisco
Period15/02/1617/02/16

Keywords

  • Optical microscopy
  • label-free imaging
  • super-resolution.

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