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Neoglycoprotein and Glycoprotein Printing on a Hydrogel Functionalized Microarray Surface and Incubation with Labeled Lectins

  • University of Galway

Research output: Chapter in Book or Conference Publication/ProceedingChapterpeer-review

Abstract

Glycan microarrays are widely used to elucidate carbohydrate binding specificity and affinity of various analytes including proteins, microorganisms, cells, and tissues. Glycan microarrays comprise a wide variety of platforms, differing in surface chemistry, presentation of carbohydrates, carbohydrate valency, and detection strategies, all of which impact on analyte performance. This chapter describes detailed methods for printing neoglycoprotein and glycoprotein microarrays on hydrogel-coated slides and incubation of these glycan microarrays with fluorescently labeled lectins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages3-24
Number of pages22
ISBN (Electronic)978-1-0716-2147-9
ISBN (Print)978-1-0716-2147-9
DOIs
Publication statusPublished - 1 Jan 2022

Publication series

NameMethods in Molecular Biology
Volume2460
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Glycan microarray
  • Glycomics microarray
  • Glycoprotein
  • Lectin profiling
  • Microarray incubation
  • Microarray printing
  • Neoglyconjugate microarray
  • Neoglycoprotein

Authors (Note for portal: view the doc link for the full list of authors)

  • Authors
  • Gerlach; JQ; Le Berre, M; Kilcoyne, M

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