Multivalent Calixarene Complexation of a Designed Pentameric Lectin

Ronan J. Flood; Linda Cerofolini; Marco Fragai; Peter B. Crowley

doi:10.1021/acs.biomac.3c01280

Multivalent Calixarene Complexation of a Designed Pentameric Lectin

Ronan J. Flood
, Linda Cerofolini
, Marco Fragai
, Peter B. Crowley

Cellular & Molecular Medicine

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

8 Citations (Scopus)

Abstract

We describe complex formation between a designed pentameric β-propeller and the anionic macrocycle sulfonato-calix[8]arene (sclx₈), as characterized by X-ray crystallography and NMR spectroscopy. Two crystal structures and ¹⁵N HSQC experiments reveal a single calixarene binding site in the concave pocket of the β-propeller toroid. Despite the symmetry mismatch between the pentameric protein and the octameric macrocycle, they form a high affinity multivalent complex, with the largest protein-calixarene interface observed to date. This system provides a platform for investigating multivalency.

Original language	English
Pages (from-to)	1303-1309
Number of pages	7
Journal	Biomacromolecules
Volume	25
Issue number	2
DOIs	https://doi.org/10.1021/acs.biomac.3c01280
Publication status	Published - 12 Feb 2024

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title = "Multivalent Calixarene Complexation of a Designed Pentameric Lectin",

abstract = "We describe complex formation between a designed pentameric β-propeller and the anionic macrocycle sulfonato-calix[8]arene (sclx8), as characterized by X-ray crystallography and NMR spectroscopy. Two crystal structures and 15N HSQC experiments reveal a single calixarene binding site in the concave pocket of the β-propeller toroid. Despite the symmetry mismatch between the pentameric protein and the octameric macrocycle, they form a high affinity multivalent complex, with the largest protein-calixarene interface observed to date. This system provides a platform for investigating multivalency.",

author = "Flood, \{Ronan J.\} and Linda Cerofolini and Marco Fragai and Crowley, \{Peter B.\}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors. Published by American Chemical Society.",

year = "2024",

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doi = "10.1021/acs.biomac.3c01280",

language = "English",

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T1 - Multivalent Calixarene Complexation of a Designed Pentameric Lectin

AU - Flood, Ronan J.

AU - Cerofolini, Linda

AU - Fragai, Marco

AU - Crowley, Peter B.

PY - 2024/2/12

Y1 - 2024/2/12

N2 - We describe complex formation between a designed pentameric β-propeller and the anionic macrocycle sulfonato-calix[8]arene (sclx8), as characterized by X-ray crystallography and NMR spectroscopy. Two crystal structures and 15N HSQC experiments reveal a single calixarene binding site in the concave pocket of the β-propeller toroid. Despite the symmetry mismatch between the pentameric protein and the octameric macrocycle, they form a high affinity multivalent complex, with the largest protein-calixarene interface observed to date. This system provides a platform for investigating multivalency.

AB - We describe complex formation between a designed pentameric β-propeller and the anionic macrocycle sulfonato-calix[8]arene (sclx8), as characterized by X-ray crystallography and NMR spectroscopy. Two crystal structures and 15N HSQC experiments reveal a single calixarene binding site in the concave pocket of the β-propeller toroid. Despite the symmetry mismatch between the pentameric protein and the octameric macrocycle, they form a high affinity multivalent complex, with the largest protein-calixarene interface observed to date. This system provides a platform for investigating multivalency.

UR - https://www.scopus.com/pages/publications/85183484303

U2 - 10.1021/acs.biomac.3c01280

DO - 10.1021/acs.biomac.3c01280

M3 - Article

SN - 1525-7797

VL - 25

SP - 1303

EP - 1309

JO - Biomacromolecules

JF - Biomacromolecules

IS - 2

ER -

Multivalent Calixarene Complexation of a Designed Pentameric Lectin

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