TY - JOUR
T1 - Multi-omics determination of metabolome diversity in natural coral populations in the Pacific Ocean
AU - Reddy, Maggie M.
AU - Goossens, Corentine
AU - Zhou, Yuxiang
AU - Chaib, Slimane
AU - Raviglione, Delphine
AU - Nicolè, Florence
AU - Hume, Benjamin C.C.
AU - Forcioli, Didier
AU - Agostini, Sylvain
AU - Boissin, Emilie
AU - Boss, Emmanuel
AU - Bowler, Chris
AU - de Vargas, Colomban
AU - Douville, Eric
AU - Flores, Michel
AU - Furla, Paola
AU - Galand, Pierre E.
AU - Gilson, Eric
AU - Lombard, Fabien
AU - Pesant, Stéphane
AU - Reynaud, Stéphanie
AU - Sullivan, Matthew B.
AU - Sunagawa, Shinichi
AU - Troublé, Romain
AU - Thurber, Rebecca Vega
AU - Wincker, Patrick
AU - Zoccola, Didier
AU - Voolstra, Christian R.
AU - Allemand, Denis
AU - Planes, Serge
AU - Thomas, Olivier P.
AU - Banaigs, Bernard
N1 - Publisher Copyright:
© 2023, Springer Nature Limited.
PY - 2023/12
Y1 - 2023/12
N2 - Coral reefs are considered one of the most emblematic ecosystems in our oceans, but their existence is increasingly threatened by climate change. In this study, natural populations of two reef-building coral genera, Pocillopora spp. and Porites spp., and one hydrocoral Millepora cf. platyphylla from two different marine provinces in the Pacific Ocean were investigated using a multi-omics approach as part of the Tara Pacific expedition. Here, we propose a standardised method consisting of a biphasic extraction method followed by metabolomics analysis using mass spectrometry for the lipidome and 1H nuclear magnetic resonance for hydrophilic metabolites. Our study assessed a broad range of the metabolome and is the first to identify and add 24 compounds by NMR and over 200 lipids by MS analyses for corals. Metabolic profiles were distinct among genera but not within genotypes of the cnidarian corals. Although endosymbiotic dinoflagellates of the family Symbiodiniaceae are known to play a central role in the metabolomic signature of the coral holobiont, they did not account for all differences. This suggests that a combined effect by different members of the coral holobiont and an interaction with the environment might be at play. Our study provides foundational knowledge on the coral holobiont metabolome.
AB - Coral reefs are considered one of the most emblematic ecosystems in our oceans, but their existence is increasingly threatened by climate change. In this study, natural populations of two reef-building coral genera, Pocillopora spp. and Porites spp., and one hydrocoral Millepora cf. platyphylla from two different marine provinces in the Pacific Ocean were investigated using a multi-omics approach as part of the Tara Pacific expedition. Here, we propose a standardised method consisting of a biphasic extraction method followed by metabolomics analysis using mass spectrometry for the lipidome and 1H nuclear magnetic resonance for hydrophilic metabolites. Our study assessed a broad range of the metabolome and is the first to identify and add 24 compounds by NMR and over 200 lipids by MS analyses for corals. Metabolic profiles were distinct among genera but not within genotypes of the cnidarian corals. Although endosymbiotic dinoflagellates of the family Symbiodiniaceae are known to play a central role in the metabolomic signature of the coral holobiont, they did not account for all differences. This suggests that a combined effect by different members of the coral holobiont and an interaction with the environment might be at play. Our study provides foundational knowledge on the coral holobiont metabolome.
UR - https://www.scopus.com/pages/publications/85168117437
U2 - 10.1038/s43247-023-00942-y
DO - 10.1038/s43247-023-00942-y
M3 - Article
AN - SCOPUS:85168117437
SN - 2662-4435
VL - 4
JO - Communications Earth and Environment
JF - Communications Earth and Environment
IS - 1
M1 - 281
ER -