TY - JOUR
T1 - Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells
AU - Yamaguchi-Iwai, Yuko
AU - Sonoda, Eiichiro
AU - Sasaki, Masao S.
AU - Morrison, Ciaran
AU - Haraguchi, Tokuko
AU - Hiraoka, Yasushi
AU - Yamashita, Yukiko M.
AU - Yagi, Takashi
AU - Takata, Minoru
AU - Price, Carolyn
AU - Kakazu, Naoki
AU - Takeda, Shunichi
PY - 1999/12/1
Y1 - 1999/12/1
N2 - Yeast Mre11 functions with Rad50 and Xrs2 in a complex that has pivotal roles in homologous recombination (HR) and non-homologous end-joining (NHEJ) DNA double-strand break (DSB) repair pathways. Vertebrate Mre11 is essential. Conditionally, MRE11 null chicken DT40 cells accumulate chromosome breaks and die upon Mre11 repression, showing frequent centrosome amplification. Mre11 deficiency also causes increased radiosensitivity and strongly reduced targeted integration frequencies. Mre11 is, therefore, crucial for HR and essential in mitosis through its role in chromosome maintenance by recombinational repair. Surprisingly perhaps, given the role of Mre11 in yeast NHEJ, disruption of NHEJ by deletion of KU70 greatly exacerbates the effects of MRE11 deficiency, revealing a significant Mre11-independent component of metazoan NHEJ.
AB - Yeast Mre11 functions with Rad50 and Xrs2 in a complex that has pivotal roles in homologous recombination (HR) and non-homologous end-joining (NHEJ) DNA double-strand break (DSB) repair pathways. Vertebrate Mre11 is essential. Conditionally, MRE11 null chicken DT40 cells accumulate chromosome breaks and die upon Mre11 repression, showing frequent centrosome amplification. Mre11 deficiency also causes increased radiosensitivity and strongly reduced targeted integration frequencies. Mre11 is, therefore, crucial for HR and essential in mitosis through its role in chromosome maintenance by recombinational repair. Surprisingly perhaps, given the role of Mre11 in yeast NHEJ, disruption of NHEJ by deletion of KU70 greatly exacerbates the effects of MRE11 deficiency, revealing a significant Mre11-independent component of metazoan NHEJ.
KW - Chromosomal aberration
KW - Double-strand break repair
KW - DT40
KW - Homologous recombination
KW - Mre11
UR - http://www.scopus.com/inward/record.url?scp=0033485758&partnerID=8YFLogxK
U2 - 10.1093/emboj/18.23.6619
DO - 10.1093/emboj/18.23.6619
M3 - Article
C2 - 10581236
AN - SCOPUS:0033485758
SN - 0261-4189
VL - 18
SP - 6619
EP - 6629
JO - EMBO Journal
JF - EMBO Journal
IS - 23
ER -