Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection

J. L.Gareta Cordero; I. K. Dimov; J. O'Grady; J. Ducréel; T. Barry; A. J. Ricco

doi:10.1109/MEMSYS.2009.4805392

Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection

J. L.Gareta Cordero, I. K. Dimov, J. O'Grady, J. Ducréel, T. Barry, A. J. Ricco

Infectious Disease

Research output: Contribution to a Journal (Peer & Non Peer) › Conference article › peer-review

1 Citation (Scopus)

Abstract

We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens. This single-cartridge platform integrates bacteria capture and concentration, supematant solution removal, lysis, and nucleic-acid sequence-based amplification (NASBA) in a single unit. The unit is fabricated using multilayer lamination and consists of five different polymer layers. Bacteria capture and concentration are accomplished by sedimentation in five minutes. Centrifugation forces also drive the subsequent steps. A wax valve is integrated in the cartridge to enable high-speed centrifugation. Oil is used to prevent evaporation during reactions requiring thermal cycling. Device functionality was demonstrated by real-time detection of E. coli from a 200-μL sample.

Original language	English
Article number	4805392
Pages (from-to)	356-359
Number of pages	4
Journal	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
DOIs	https://doi.org/10.1109/MEMSYS.2009.4805392
Publication status	Published - 2009
Event	22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy Duration: 25 Jan 2009 → 29 Jan 2009

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Cordero, J. L. G., Dimov, I. K., O'Grady, J., Ducréel, J., Barry, T., & Ricco, A. J. (2009). Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 356-359. Article 4805392. https://doi.org/10.1109/MEMSYS.2009.4805392

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title = "Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection",

abstract = "We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens. This single-cartridge platform integrates bacteria capture and concentration, supematant solution removal, lysis, and nucleic-acid sequence-based amplification (NASBA) in a single unit. The unit is fabricated using multilayer lamination and consists of five different polymer layers. Bacteria capture and concentration are accomplished by sedimentation in five minutes. Centrifugation forces also drive the subsequent steps. A wax valve is integrated in the cartridge to enable high-speed centrifugation. Oil is used to prevent evaporation during reactions requiring thermal cycling. Device functionality was demonstrated by real-time detection of E. coli from a 200-μL sample.",

author = "Cordero, {J. L.Gareta} and Dimov, {I. K.} and J. O'Grady and J. Ducr{\'e}el and T. Barry and Ricco, {A. J.}",

year = "2009",

doi = "10.1109/MEMSYS.2009.4805392",

language = "English",

pages = "356--359",

journal = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

issn = "1084-6999",

note = "22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 ; Conference date: 25-01-2009 Through 29-01-2009",

}

Cordero, JLG, Dimov, IK, O'Grady, J, Ducréel, J, Barry, T & Ricco, AJ 2009, 'Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection', Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 356-359. https://doi.org/10.1109/MEMSYS.2009.4805392

Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection. / Cordero, J. L.Gareta; Dimov, I. K.; O'Grady, J. et al.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2009, p. 356-359.

Research output: Contribution to a Journal (Peer & Non Peer) › Conference article › peer-review

TY - JOUR

T1 - Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection

AU - Cordero, J. L.Gareta

AU - Dimov, I. K.

AU - O'Grady, J.

AU - Ducréel, J.

AU - Barry, T.

AU - Ricco, A. J.

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N2 - We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens. This single-cartridge platform integrates bacteria capture and concentration, supematant solution removal, lysis, and nucleic-acid sequence-based amplification (NASBA) in a single unit. The unit is fabricated using multilayer lamination and consists of five different polymer layers. Bacteria capture and concentration are accomplished by sedimentation in five minutes. Centrifugation forces also drive the subsequent steps. A wax valve is integrated in the cartridge to enable high-speed centrifugation. Oil is used to prevent evaporation during reactions requiring thermal cycling. Device functionality was demonstrated by real-time detection of E. coli from a 200-μL sample.

AB - We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens. This single-cartridge platform integrates bacteria capture and concentration, supematant solution removal, lysis, and nucleic-acid sequence-based amplification (NASBA) in a single unit. The unit is fabricated using multilayer lamination and consists of five different polymer layers. Bacteria capture and concentration are accomplished by sedimentation in five minutes. Centrifugation forces also drive the subsequent steps. A wax valve is integrated in the cartridge to enable high-speed centrifugation. Oil is used to prevent evaporation during reactions requiring thermal cycling. Device functionality was demonstrated by real-time detection of E. coli from a 200-μL sample.

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DO - 10.1109/MEMSYS.2009.4805392

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AN - SCOPUS:65949105514

SN - 1084-6999

SP - 356

EP - 359

JO - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

JF - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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T2 - 22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009

Y2 - 25 January 2009 through 29 January 2009

ER -

Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection

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