Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization

Amaia Sangrador-Vegas; Jessica B. Lennington; Terry J. Smith

doi:10.1006/cyto.2001.0991

Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization

Amaia Sangrador-Vegas, Jessica B. Lennington, Terry J. Smith

Infectious Disease

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

67 Citations (Scopus)

Abstract

Suppression subtractive hybridization (SSH) was performed to construct a Rainbow trout cDNA library enriched in sequences up-regulated in head kidney leukocytes after lipopolysaccharide (LPS) and tumour necrosis factor alpha (TNFα) stimulation. Random sequencing of fifty clones allowed the identification of a Rainbow trout interleukin 8 (IL-8)-related CXC chemokine, as well as the Rainbow trout tissue factor (TF) precursor. Expression of both the IL-8-like chemokine and TF is induced after LPS and TNFα stimulation, indicating that they are associated with inflammatory responses in fish, as has been suggested in mammals. These results confirm the potential of SSH to identify cytokines and immuno-regulatory genes in fish.

Original language	English
Pages (from-to)	66-70
Number of pages	5
Journal	Cytokine
Volume	17
Issue number	2
DOIs	https://doi.org/10.1006/cyto.2001.0991
Publication status	Published - 2002

Keywords

Chemokine
Immune-stimulation
SSH
Teleosts
Tissue factor

Access to Document

10.1006/cyto.2001.0991

Cite this

@article{383fa9c8578f4a389bd6c42ab05ec8c5,

title = "Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization",

abstract = "Suppression subtractive hybridization (SSH) was performed to construct a Rainbow trout cDNA library enriched in sequences up-regulated in head kidney leukocytes after lipopolysaccharide (LPS) and tumour necrosis factor alpha (TNFα) stimulation. Random sequencing of fifty clones allowed the identification of a Rainbow trout interleukin 8 (IL-8)-related CXC chemokine, as well as the Rainbow trout tissue factor (TF) precursor. Expression of both the IL-8-like chemokine and TF is induced after LPS and TNFα stimulation, indicating that they are associated with inflammatory responses in fish, as has been suggested in mammals. These results confirm the potential of SSH to identify cytokines and immuno-regulatory genes in fish.",

keywords = "Chemokine, Immune-stimulation, SSH, Teleosts, Tissue factor",

author = "Amaia Sangrador-Vegas and Lennington, \{Jessica B.\} and Smith, \{Terry J.\}",

year = "2002",

doi = "10.1006/cyto.2001.0991",

language = "English",

volume = "17",

pages = "66--70",

journal = "Cytokine",

issn = "1043-4666",

publisher = "Academic Press",

number = "2",

}

TY - JOUR

T1 - Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization

AU - Sangrador-Vegas, Amaia

AU - Lennington, Jessica B.

AU - Smith, Terry J.

PY - 2002

Y1 - 2002

N2 - Suppression subtractive hybridization (SSH) was performed to construct a Rainbow trout cDNA library enriched in sequences up-regulated in head kidney leukocytes after lipopolysaccharide (LPS) and tumour necrosis factor alpha (TNFα) stimulation. Random sequencing of fifty clones allowed the identification of a Rainbow trout interleukin 8 (IL-8)-related CXC chemokine, as well as the Rainbow trout tissue factor (TF) precursor. Expression of both the IL-8-like chemokine and TF is induced after LPS and TNFα stimulation, indicating that they are associated with inflammatory responses in fish, as has been suggested in mammals. These results confirm the potential of SSH to identify cytokines and immuno-regulatory genes in fish.

AB - Suppression subtractive hybridization (SSH) was performed to construct a Rainbow trout cDNA library enriched in sequences up-regulated in head kidney leukocytes after lipopolysaccharide (LPS) and tumour necrosis factor alpha (TNFα) stimulation. Random sequencing of fifty clones allowed the identification of a Rainbow trout interleukin 8 (IL-8)-related CXC chemokine, as well as the Rainbow trout tissue factor (TF) precursor. Expression of both the IL-8-like chemokine and TF is induced after LPS and TNFα stimulation, indicating that they are associated with inflammatory responses in fish, as has been suggested in mammals. These results confirm the potential of SSH to identify cytokines and immuno-regulatory genes in fish.

KW - Chemokine

KW - Immune-stimulation

KW - SSH

KW - Teleosts

KW - Tissue factor

UR - https://www.scopus.com/pages/publications/0036058351

U2 - 10.1006/cyto.2001.0991

DO - 10.1006/cyto.2001.0991

M3 - Article

SN - 1043-4666

VL - 17

SP - 66

EP - 70

JO - Cytokine

JF - Cytokine

IS - 2

ER -

Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this