The orientation of collagen fibres in bovine articular cartilage was analyzed using polarized light microscopy (PLM). Cartilage samples from four bovine tibial plateaux were obtained from a local abattoir and were harvested. The samples were fixed in 0.5% glutaraldehyde in a 0.0067 M phosphate buffer solution at room temperature for 24 hours. The samples were mounted on a glass slides and analyzed under a polarized light microscope. It was observed that 77% of the fibres were oriented in the vertical direction between the angles of 45°and 135°, while 23% of the fibres were oriented in the horizontal position between 0°-45° and 135°-180°.