Methods for the detection of apoptosis and necrosis

Afshin Samali

doi:10.1002/0471140856.tx0202s00

Methods for the detection of apoptosis and necrosis

Afshin Samali

Cellular & Molecular Medicine

Research output: Chapter in Book or Conference Publication/Proceeding › Chapter › peer-review

Abstract

Cell death occurs by one of two mechanisms: apoptosis (programmed cell death) or necrosis due to cellular injury or trauma. Each method has distinct morphological and biochemical characteristics. Distinguishing between apoptosis and necrosis depends selection of appropriate assays and proper timing. This unit provides an extensive series of assays that measure membrane integrity, cell size and DNA content, state of chromatin, DNA fragmentation, and caspase activation. The results of these assays allow one to distinguish between apoptosis and necrosis.

Original language	English (Ireland)
Title of host publication	Current Protocols in Toxicology
Publisher	John Wiley and Sons, Inc.
DOIs	https://doi.org/10.1002/0471140856.tx0202s00
Publication status	Published - 1 Jan 1999

Authors (Note for portal: view the doc link for the full list of authors)

Authors
Zhivotovsky B., Samali A., Orrenius S.

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10.1002/0471140856.tx0202s00

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title = "Methods for the detection of apoptosis and necrosis",

abstract = "Cell death occurs by one of two mechanisms: apoptosis (programmed cell death) or necrosis due to cellular injury or trauma. Each method has distinct morphological and biochemical characteristics. Distinguishing between apoptosis and necrosis depends selection of appropriate assays and proper timing. This unit provides an extensive series of assays that measure membrane integrity, cell size and DNA content, state of chromatin, DNA fragmentation, and caspase activation. The results of these assays allow one to distinguish between apoptosis and necrosis.",

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year = "1999",

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N2 - Cell death occurs by one of two mechanisms: apoptosis (programmed cell death) or necrosis due to cellular injury or trauma. Each method has distinct morphological and biochemical characteristics. Distinguishing between apoptosis and necrosis depends selection of appropriate assays and proper timing. This unit provides an extensive series of assays that measure membrane integrity, cell size and DNA content, state of chromatin, DNA fragmentation, and caspase activation. The results of these assays allow one to distinguish between apoptosis and necrosis.

AB - Cell death occurs by one of two mechanisms: apoptosis (programmed cell death) or necrosis due to cellular injury or trauma. Each method has distinct morphological and biochemical characteristics. Distinguishing between apoptosis and necrosis depends selection of appropriate assays and proper timing. This unit provides an extensive series of assays that measure membrane integrity, cell size and DNA content, state of chromatin, DNA fragmentation, and caspase activation. The results of these assays allow one to distinguish between apoptosis and necrosis.

U2 - 10.1002/0471140856.tx0202s00

DO - 10.1002/0471140856.tx0202s00

M3 - Chapter

BT - Current Protocols in Toxicology

PB - John Wiley and Sons, Inc.

ER -

Methods for the detection of apoptosis and necrosis

Abstract

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