Measurement of hepatobiliary function and hepatic hemodynamics in portally hypertensive rats

A. M. Wheatley; D. Zhao; F. G. Höflin; E. T. Stuart; T. Guastella; A. Czerniak; L. H. Blumgart

doi:10.1007/BF02602091

Measurement of hepatobiliary function and hepatic hemodynamics in portally hypertensive rats

A. M. Wheatley
, D. Zhao
, F. G. Höflin
, E. T. Stuart
, T. Guastella
, A. Czerniak
, L. H. Blumgart

University of Bern

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

1 Citation (Scopus)

Abstract

Background: Portal hypertension induced by partial ligation of the portal vein (PPVL) is associated with cardiovascular changes including portal systemic shunting (PSS). Despite large diversion of portal blood, there are no reports on the effect of PPVL on hepatobiliary function. Here we report on the effect of PPVL on liver function. Methods: Male Lewis rats were divided into 3 groups: control (n=10), PPVL (n=10) and bile duct ligated (BDL) (n=4). Under anesthesia, PPVL was performed around a 21-gauge needle and BDL was by ligation of the common bile duct. Under ether anesthesia, 0.1 mCi^99mTc-labelled Hepatoiodida^® was injected via the penile vein and scintigraphy performed. The heart and liver were chosen as regions of interest and the mean transit times for the heart (MTT_heart) and liver (MTT_liver) were calculated. PSS was measured by direct intraportal injection of⁵⁷Co-labelled microspheres. Results: Portal pressure was significantly elevated in the PPVL group (p<0.001 vs. control) and PSS was in evidence (24±15%, SD, p<0,001). The MTT_heart and MTT_liver for the control group (97±12 sec and 357±49 sec, respectively) were not significantly different than those for the PPVL group (150±50 sec and 410±48 sec, respectively). In the BDL group, the MTT_heart and MTT_liver were significantly different from control and PPVL. Conclusions: We conclude from our results that despite the presence of significant PSS, no change in hepatobiliary function in rats with prehepatic portal hypertension occurs.

Original language	English
Pages (from-to)	174-178
Number of pages	5
Journal	Acta Chirurgica Austriaca
Volume	25
Issue number	3
DOIs	https://doi.org/10.1007/BF02602091
Publication status	Published - May 1993
Externally published	Yes

Keywords

bile duct ligation
hepatic function
hepatic microcirculation
Portal hypertension
portal systemic shunt
rat

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10.1007/BF02602091

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@article{5c6ce7f861b94a7280154b9e0857386a,

title = "Measurement of hepatobiliary function and hepatic hemodynamics in portally hypertensive rats",

abstract = "Background: Portal hypertension induced by partial ligation of the portal vein (PPVL) is associated with cardiovascular changes including portal systemic shunting (PSS). Despite large diversion of portal blood, there are no reports on the effect of PPVL on hepatobiliary function. Here we report on the effect of PPVL on liver function. Methods: Male Lewis rats were divided into 3 groups: control (n=10), PPVL (n=10) and bile duct ligated (BDL) (n=4). Under anesthesia, PPVL was performed around a 21-gauge needle and BDL was by ligation of the common bile duct. Under ether anesthesia, 0.1 mCi99mTc-labelled Hepatoiodida{\textregistered} was injected via the penile vein and scintigraphy performed. The heart and liver were chosen as regions of interest and the mean transit times for the heart (MTTheart) and liver (MTTliver) were calculated. PSS was measured by direct intraportal injection of57Co-labelled microspheres. Results: Portal pressure was significantly elevated in the PPVL group (p<0.001 vs. control) and PSS was in evidence (24±15\%, SD, p<0,001). The MTTheart and MTTliver for the control group (97±12 sec and 357±49 sec, respectively) were not significantly different than those for the PPVL group (150±50 sec and 410±48 sec, respectively). In the BDL group, the MTTheart and MTTliver were significantly different from control and PPVL. Conclusions: We conclude from our results that despite the presence of significant PSS, no change in hepatobiliary function in rats with prehepatic portal hypertension occurs.",

keywords = "bile duct ligation, hepatic function, hepatic microcirculation, Portal hypertension, portal systemic shunt, rat",

author = "Wheatley, \{A. M.\} and D. Zhao and H{\"o}flin, \{F. G.\} and Stuart, \{E. T.\} and T. Guastella and A. Czerniak and Blumgart, \{L. H.\}",

year = "1993",

month = may,

doi = "10.1007/BF02602091",

language = "English",

volume = "25",

pages = "174--178",

journal = "Acta Chirurgica Austriaca",

issn = "0001-544X",

publisher = "Blackwell Verlag Wien",

number = "3",

}

TY - JOUR

T1 - Measurement of hepatobiliary function and hepatic hemodynamics in portally hypertensive rats

AU - Wheatley, A. M.

AU - Zhao, D.

AU - Höflin, F. G.

AU - Stuart, E. T.

AU - Guastella, T.

AU - Czerniak, A.

AU - Blumgart, L. H.

PY - 1993/5

Y1 - 1993/5

N2 - Background: Portal hypertension induced by partial ligation of the portal vein (PPVL) is associated with cardiovascular changes including portal systemic shunting (PSS). Despite large diversion of portal blood, there are no reports on the effect of PPVL on hepatobiliary function. Here we report on the effect of PPVL on liver function. Methods: Male Lewis rats were divided into 3 groups: control (n=10), PPVL (n=10) and bile duct ligated (BDL) (n=4). Under anesthesia, PPVL was performed around a 21-gauge needle and BDL was by ligation of the common bile duct. Under ether anesthesia, 0.1 mCi99mTc-labelled Hepatoiodida® was injected via the penile vein and scintigraphy performed. The heart and liver were chosen as regions of interest and the mean transit times for the heart (MTTheart) and liver (MTTliver) were calculated. PSS was measured by direct intraportal injection of57Co-labelled microspheres. Results: Portal pressure was significantly elevated in the PPVL group (p<0.001 vs. control) and PSS was in evidence (24±15%, SD, p<0,001). The MTTheart and MTTliver for the control group (97±12 sec and 357±49 sec, respectively) were not significantly different than those for the PPVL group (150±50 sec and 410±48 sec, respectively). In the BDL group, the MTTheart and MTTliver were significantly different from control and PPVL. Conclusions: We conclude from our results that despite the presence of significant PSS, no change in hepatobiliary function in rats with prehepatic portal hypertension occurs.

AB - Background: Portal hypertension induced by partial ligation of the portal vein (PPVL) is associated with cardiovascular changes including portal systemic shunting (PSS). Despite large diversion of portal blood, there are no reports on the effect of PPVL on hepatobiliary function. Here we report on the effect of PPVL on liver function. Methods: Male Lewis rats were divided into 3 groups: control (n=10), PPVL (n=10) and bile duct ligated (BDL) (n=4). Under anesthesia, PPVL was performed around a 21-gauge needle and BDL was by ligation of the common bile duct. Under ether anesthesia, 0.1 mCi99mTc-labelled Hepatoiodida® was injected via the penile vein and scintigraphy performed. The heart and liver were chosen as regions of interest and the mean transit times for the heart (MTTheart) and liver (MTTliver) were calculated. PSS was measured by direct intraportal injection of57Co-labelled microspheres. Results: Portal pressure was significantly elevated in the PPVL group (p<0.001 vs. control) and PSS was in evidence (24±15%, SD, p<0,001). The MTTheart and MTTliver for the control group (97±12 sec and 357±49 sec, respectively) were not significantly different than those for the PPVL group (150±50 sec and 410±48 sec, respectively). In the BDL group, the MTTheart and MTTliver were significantly different from control and PPVL. Conclusions: We conclude from our results that despite the presence of significant PSS, no change in hepatobiliary function in rats with prehepatic portal hypertension occurs.

KW - bile duct ligation

KW - hepatic function

KW - hepatic microcirculation

KW - Portal hypertension

KW - portal systemic shunt

KW - rat

UR - https://www.scopus.com/pages/publications/0027306833

U2 - 10.1007/BF02602091

DO - 10.1007/BF02602091

M3 - Article

AN - SCOPUS:0027306833

SN - 0001-544X

VL - 25

SP - 174

EP - 178

JO - Acta Chirurgica Austriaca

JF - Acta Chirurgica Austriaca

IS - 3

ER -

Measurement of hepatobiliary function and hepatic hemodynamics in portally hypertensive rats

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