Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation

Tadhg Griffin; Klaus Mosbach; Rolf Mosbach

doi:10.1007/BF02798279

Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation

Tadhg Griffin
, Klaus Mosbach
, Rolf Mosbach

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

17 Citations (Scopus)

Abstract

Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation have been prepared. They were obtained by a " post-magnetization" procedure involving a simple treatment of the various affinity gels with magnetic ferrofluid. The magnetic biospecific adsorbents tested include magnetic protein A-Sepharose for isolation of IgG antibodies, magnetic human serum albumin (HSA)-Sepharose for anti-HSA isolation, and magnetic 2′,5′-ADP for isolation of glucose-6-phosphate dehydrogenase from baker's yeast and hemolyzates of human red blood cells. For the latter enzyme, a 11,000-fold purification was achieved in one step.

Original language	English
Pages (from-to)	283-292
Number of pages	10
Journal	Applied Biochemistry and Biotechnology
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/BF02798279
Publication status	Published - Dec 1981
Externally published	Yes

Keywords

adsorbents, magnetic biospecific affinity
affinity adsorbents, magnetic biospecific
biospecific affinity adsorbents, magnetic
enzymes, isolation by magnetic affinity adsorbents
glucose-6-phosphate dehydrogenase, isolation by magnetic affinity adsorbents
immunoglobulin, isolation by magnetic affinity adsorbents
Magnetic biospecific affinity adsorbents

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10.1007/BF02798279

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title = "Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation",

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author = "Tadhg Griffin and Klaus Mosbach and Rolf Mosbach",

year = "1981",

month = dec,

doi = "10.1007/BF02798279",

language = "English",

volume = "6",

pages = "283--292",

journal = "Applied Biochemistry and Biotechnology",

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TY - JOUR

T1 - Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation

AU - Griffin, Tadhg

AU - Mosbach, Klaus

AU - Mosbach, Rolf

PY - 1981/12

Y1 - 1981/12

N2 - Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation have been prepared. They were obtained by a " post-magnetization" procedure involving a simple treatment of the various affinity gels with magnetic ferrofluid. The magnetic biospecific adsorbents tested include magnetic protein A-Sepharose for isolation of IgG antibodies, magnetic human serum albumin (HSA)-Sepharose for anti-HSA isolation, and magnetic 2′,5′-ADP for isolation of glucose-6-phosphate dehydrogenase from baker's yeast and hemolyzates of human red blood cells. For the latter enzyme, a 11,000-fold purification was achieved in one step.

AB - Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation have been prepared. They were obtained by a " post-magnetization" procedure involving a simple treatment of the various affinity gels with magnetic ferrofluid. The magnetic biospecific adsorbents tested include magnetic protein A-Sepharose for isolation of IgG antibodies, magnetic human serum albumin (HSA)-Sepharose for anti-HSA isolation, and magnetic 2′,5′-ADP for isolation of glucose-6-phosphate dehydrogenase from baker's yeast and hemolyzates of human red blood cells. For the latter enzyme, a 11,000-fold purification was achieved in one step.

KW - adsorbents, magnetic biospecific affinity

KW - affinity adsorbents, magnetic biospecific

KW - biospecific affinity adsorbents, magnetic

KW - enzymes, isolation by magnetic affinity adsorbents

KW - glucose-6-phosphate dehydrogenase, isolation by magnetic affinity adsorbents

KW - immunoglobulin, isolation by magnetic affinity adsorbents

KW - Magnetic biospecific affinity adsorbents

UR - https://www.scopus.com/pages/publications/0019768807

U2 - 10.1007/BF02798279

DO - 10.1007/BF02798279

M3 - Article

AN - SCOPUS:0019768807

SN - 0273-2289

VL - 6

SP - 283

EP - 292

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 4

ER -

Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation

Abstract

Keywords

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