TY - JOUR
T1 - Lymphoproliferative disorders in IL-7 transgenic mice
T2 - Expansion of immature B cells which retain macrophage potential
AU - Fisher, A. G.
AU - Burdet, C.
AU - Bunce, C.
AU - Merkenschlager, M.
AU - Ceredig, Rh
PY - 1995/3
Y1 - 1995/3
N2 - Transgenic mice carrying the murine IL-7 gene under the MHC class II (Eα) promoter are described which develop lymphoid tumours at a high incidence when maintained in conventional or specific pathogen-free environments. Cells obtained from the lesions were relatively monomorphic, expressed a variety of B cell associated markers (BP-1, B220, CD43) but lacked surface Ig. Some mice showed expanded populations of cells phenotypically similar to the recently reported bipotent B/macrophage stem cell subset (AA4.1high, B220-, Ig-) which could be cloned and maintained in vitro. These cells expressed IL-7 receptors, proliferated in response to IL-7 and in most cases had germllne configuration of the Ig heavy chain locus. Cell lines cloned from two such tumours generated macrophages spontaneously in culture, consistent with their bipotent B cell/macrophage phenotype. These results suggest that IL-7 plays a role in very early stages of B cell ontogeny prior to bona fide B cell commitment.
AB - Transgenic mice carrying the murine IL-7 gene under the MHC class II (Eα) promoter are described which develop lymphoid tumours at a high incidence when maintained in conventional or specific pathogen-free environments. Cells obtained from the lesions were relatively monomorphic, expressed a variety of B cell associated markers (BP-1, B220, CD43) but lacked surface Ig. Some mice showed expanded populations of cells phenotypically similar to the recently reported bipotent B/macrophage stem cell subset (AA4.1high, B220-, Ig-) which could be cloned and maintained in vitro. These cells expressed IL-7 receptors, proliferated in response to IL-7 and in most cases had germllne configuration of the Ig heavy chain locus. Cell lines cloned from two such tumours generated macrophages spontaneously in culture, consistent with their bipotent B cell/macrophage phenotype. These results suggest that IL-7 plays a role in very early stages of B cell ontogeny prior to bona fide B cell commitment.
KW - B cell
KW - IL-7
KW - Lymphoproliferative disorder
KW - Macrophage
UR - https://www.scopus.com/pages/publications/0028924203
U2 - 10.1093/intimm/7.3.415
DO - 10.1093/intimm/7.3.415
M3 - Article
SN - 0953-8178
VL - 7
SP - 415
EP - 423
JO - International Immunology
JF - International Immunology
IS - 3
ER -
