Luminescent whole-cell cyanobacterial bioreporter for measuring Fe availability in diverse marine environments

Ramakrishna Boyanapalli; George S. Bullerjahn; Christa Pohl; Peter L. Croot; Philip W. Boyd; R. Michael L. McKay

doi:10.1128/AEM.01670-06

Luminescent whole-cell cyanobacterial bioreporter for measuring Fe availability in diverse marine environments

Ramakrishna Boyanapalli
, George S. Bullerjahn
, Christa Pohl
, Peter L. Croot
, Philip W. Boyd
, R. Michael L. McKay

Bowling Green State University
University of Colorado Boulder
Baltic Sea Research Institute
Marine Natural Products Chemistry
University of Otago

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

45 Citations (Scopus)

Abstract

A Synechococcus sp. strain PCC 7002 Fe bioreporter was constructed containing the isiAB promoter fused to the Vibrio harveyi luxAB genes. Bioreporter luminescence was characterized with respect to the free ferric ion concentration in trace metal-buffered synthetic medium. The applicability of the Fe bioreporter to assess Fe availability in the natural environment was tested by using samples collected from the Baltic Sea and from the high-nutrient, low-chlorophyll subarctic Pacific Ocean. Parallel assessment of dissolved Fe and bioreporter response confirmed that direct chemical measurements of dissolved Fe should not be considered alone when assessing Fe availability to phytoplankton.

Original language	English
Pages (from-to)	1019-1024
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	73
Issue number	3
DOIs	https://doi.org/10.1128/AEM.01670-06
Publication status	Published - Feb 2007
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

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10.1128/AEM.01670-06

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title = "Luminescent whole-cell cyanobacterial bioreporter for measuring Fe availability in diverse marine environments",

abstract = "A Synechococcus sp. strain PCC 7002 Fe bioreporter was constructed containing the isiAB promoter fused to the Vibrio harveyi luxAB genes. Bioreporter luminescence was characterized with respect to the free ferric ion concentration in trace metal-buffered synthetic medium. The applicability of the Fe bioreporter to assess Fe availability in the natural environment was tested by using samples collected from the Baltic Sea and from the high-nutrient, low-chlorophyll subarctic Pacific Ocean. Parallel assessment of dissolved Fe and bioreporter response confirmed that direct chemical measurements of dissolved Fe should not be considered alone when assessing Fe availability to phytoplankton.",

author = "Ramakrishna Boyanapalli and Bullerjahn, \{George S.\} and Christa Pohl and Croot, \{Peter L.\} and Boyd, \{Philip W.\} and McKay, \{R. Michael L.\}",

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doi = "10.1128/AEM.01670-06",

language = "English",

volume = "73",

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AU - Boyanapalli, Ramakrishna

AU - Bullerjahn, George S.

AU - Pohl, Christa

AU - Croot, Peter L.

AU - Boyd, Philip W.

AU - McKay, R. Michael L.

PY - 2007/2

Y1 - 2007/2

N2 - A Synechococcus sp. strain PCC 7002 Fe bioreporter was constructed containing the isiAB promoter fused to the Vibrio harveyi luxAB genes. Bioreporter luminescence was characterized with respect to the free ferric ion concentration in trace metal-buffered synthetic medium. The applicability of the Fe bioreporter to assess Fe availability in the natural environment was tested by using samples collected from the Baltic Sea and from the high-nutrient, low-chlorophyll subarctic Pacific Ocean. Parallel assessment of dissolved Fe and bioreporter response confirmed that direct chemical measurements of dissolved Fe should not be considered alone when assessing Fe availability to phytoplankton.

AB - A Synechococcus sp. strain PCC 7002 Fe bioreporter was constructed containing the isiAB promoter fused to the Vibrio harveyi luxAB genes. Bioreporter luminescence was characterized with respect to the free ferric ion concentration in trace metal-buffered synthetic medium. The applicability of the Fe bioreporter to assess Fe availability in the natural environment was tested by using samples collected from the Baltic Sea and from the high-nutrient, low-chlorophyll subarctic Pacific Ocean. Parallel assessment of dissolved Fe and bioreporter response confirmed that direct chemical measurements of dissolved Fe should not be considered alone when assessing Fe availability to phytoplankton.

UR - https://www.scopus.com/pages/publications/33846940934

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DO - 10.1128/AEM.01670-06

M3 - Article

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VL - 73

SP - 1019

EP - 1024

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 3

ER -

Luminescent whole-cell cyanobacterial bioreporter for measuring Fe availability in diverse marine environments

Abstract

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