Low oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human corneal fibroblast culture

Pramod Kumar; Abhigyan Satyam; Daniela Cigognini; Abhay Pandit; Dimitrios I. Zeugolis

doi:10.1002/term.2283

Low oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human corneal fibroblast culture

Pramod Kumar
, Abhigyan Satyam
, Daniela Cigognini
, Abhay Pandit
, Dimitrios I. Zeugolis

Mechanical Engineering

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

48 Citations (Scopus)

Abstract

Development of implantable devices based on the principles of in vitro organogenesis has been hindered due to the prolonged time required to develop an implantable device. Herein we assessed the influence of serum concentration (0.5% and 10%), oxygen tension (0.5%, 2% and 20%) and macromolecular crowding (75 μg/ml carrageenan) in extracellular matrix deposition in human corneal fibroblast culture (3, 7 and 14 days). The highest extracellular matrix deposition was observed after 14 days in culture at 0.5% serum, 2% oxygen tension and 75 μg/ml carrageenan. These data indicate that low oxygen tension coupled with macromolecular crowding significantly accelerate the development of scaffold-free tissue-like modules.

Original language	English
Pages (from-to)	6-18
Number of pages	13
Journal	Journal of Tissue Engineering and Regenerative Medicine
Volume	12
Issue number	1
DOIs	https://doi.org/10.1002/term.2283
Publication status	Published - 1 Jan 2018

Keywords

Cell therapies
Corneal tissue engineering
Excluding volume effect
Hypoxia
In vitro microenvironment
In vitro organogenesis

Authors (Note for portal: view the doc link for the full list of authors)

Authors
Kumar, P,Satyam, A,Cigognini, D,Pandit, A,Zeugolis, DI

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10.1002/term.2283

Cite this

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title = "Low oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human corneal fibroblast culture",

abstract = "Development of implantable devices based on the principles of in vitro organogenesis has been hindered due to the prolonged time required to develop an implantable device. Herein we assessed the influence of serum concentration (0.5\% and 10\%), oxygen tension (0.5\%, 2\% and 20\%) and macromolecular crowding (75 μg/ml carrageenan) in extracellular matrix deposition in human corneal fibroblast culture (3, 7 and 14 days). The highest extracellular matrix deposition was observed after 14 days in culture at 0.5\% serum, 2\% oxygen tension and 75 μg/ml carrageenan. These data indicate that low oxygen tension coupled with macromolecular crowding significantly accelerate the development of scaffold-free tissue-like modules.",

keywords = "Cell therapies, Corneal tissue engineering, Excluding volume effect, Hypoxia, In vitro microenvironment, In vitro organogenesis",

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doi = "10.1002/term.2283",

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Low oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human corneal fibroblast culture. / Kumar, Pramod; Satyam, Abhigyan; Cigognini, Daniela et al.
In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 1, 01.01.2018, p. 6-18.

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

TY - JOUR

T1 - Low oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human corneal fibroblast culture

AU - Kumar, Pramod

AU - Satyam, Abhigyan

AU - Cigognini, Daniela

AU - Pandit, Abhay

AU - Zeugolis, Dimitrios I.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Development of implantable devices based on the principles of in vitro organogenesis has been hindered due to the prolonged time required to develop an implantable device. Herein we assessed the influence of serum concentration (0.5% and 10%), oxygen tension (0.5%, 2% and 20%) and macromolecular crowding (75 μg/ml carrageenan) in extracellular matrix deposition in human corneal fibroblast culture (3, 7 and 14 days). The highest extracellular matrix deposition was observed after 14 days in culture at 0.5% serum, 2% oxygen tension and 75 μg/ml carrageenan. These data indicate that low oxygen tension coupled with macromolecular crowding significantly accelerate the development of scaffold-free tissue-like modules.

AB - Development of implantable devices based on the principles of in vitro organogenesis has been hindered due to the prolonged time required to develop an implantable device. Herein we assessed the influence of serum concentration (0.5% and 10%), oxygen tension (0.5%, 2% and 20%) and macromolecular crowding (75 μg/ml carrageenan) in extracellular matrix deposition in human corneal fibroblast culture (3, 7 and 14 days). The highest extracellular matrix deposition was observed after 14 days in culture at 0.5% serum, 2% oxygen tension and 75 μg/ml carrageenan. These data indicate that low oxygen tension coupled with macromolecular crowding significantly accelerate the development of scaffold-free tissue-like modules.

KW - Cell therapies

KW - Corneal tissue engineering

KW - Excluding volume effect

KW - Hypoxia

KW - In vitro microenvironment

KW - In vitro organogenesis

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U2 - 10.1002/term.2283

DO - 10.1002/term.2283

M3 - Article

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ER -

Low oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human corneal fibroblast culture

Abstract

Keywords

Authors (Note for portal: view the doc link for the full list of authors)

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