Isolation and functional analysis of the histone H3 promoter from Atlantic salmon (Salmo salar L.)

Sean Hanley; Terry J. Smith; Ferenc Muller; Norman Maclean; Svetlana Uzbekova; Patrick Prunet; Bernard Breton

Isolation and functional analysis of the histone H3 promoter from Atlantic salmon (Salmo salar L.)

Sean Hanley, Terry J. Smith, Ferenc Muller, Norman Maclean, Svetlana Uzbekova, Patrick Prunet, Bernard Breton

Infectious Disease

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

16 Citations (Scopus)

Abstract

The histone H3 (sH3) promoter of Atlantic salmon (Salmo salar) was cloned via polymerase chain reaction using primers designed from the rainbow trout (Oncorhynchus mykiss) promoter sequence. A comparison of the nucleotide sequence with the equivalent sequences from rainbow trout and sockeye salmon (Oncorhynchus nerka) revealed a high degree of conservation. In vivo expression analysis of the sH3 promoter was carried out in both rainbow trout and zebra fish (Danio rerio) embryos. A direct comparison of the sH3 promoter with the viral RSV promoter in rainbow trout resulted in stronger expression of the sH3 promoter. Furthermore, lacZ expression directed by the sH3 promoter was ubiquitous in several different cell types in developing zebra fish embryos. These results suggest that the sH3 promoter will be useful in transgenic studies in Atlantic salmon.

Original language	English
Pages (from-to)	165-172
Number of pages	8
Journal	Molecular marine biology and biotechnology
Volume	7
Issue number	3
Publication status	Published - Sep 1998

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title = "Isolation and functional analysis of the histone H3 promoter from Atlantic salmon (Salmo salar L.)",

abstract = "The histone H3 (sH3) promoter of Atlantic salmon (Salmo salar) was cloned via polymerase chain reaction using primers designed from the rainbow trout (Oncorhynchus mykiss) promoter sequence. A comparison of the nucleotide sequence with the equivalent sequences from rainbow trout and sockeye salmon (Oncorhynchus nerka) revealed a high degree of conservation. In vivo expression analysis of the sH3 promoter was carried out in both rainbow trout and zebra fish (Danio rerio) embryos. A direct comparison of the sH3 promoter with the viral RSV promoter in rainbow trout resulted in stronger expression of the sH3 promoter. Furthermore, lacZ expression directed by the sH3 promoter was ubiquitous in several different cell types in developing zebra fish embryos. These results suggest that the sH3 promoter will be useful in transgenic studies in Atlantic salmon.",

author = "Sean Hanley and Smith, \{Terry J.\} and Ferenc Muller and Norman Maclean and Svetlana Uzbekova and Patrick Prunet and Bernard Breton",

year = "1998",

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language = "English",

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pages = "165--172",

journal = "Molecular marine biology and biotechnology",

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T1 - Isolation and functional analysis of the histone H3 promoter from Atlantic salmon (Salmo salar L.)

AU - Hanley, Sean

AU - Smith, Terry J.

AU - Muller, Ferenc

AU - Maclean, Norman

AU - Uzbekova, Svetlana

AU - Prunet, Patrick

AU - Breton, Bernard

PY - 1998/9

Y1 - 1998/9

N2 - The histone H3 (sH3) promoter of Atlantic salmon (Salmo salar) was cloned via polymerase chain reaction using primers designed from the rainbow trout (Oncorhynchus mykiss) promoter sequence. A comparison of the nucleotide sequence with the equivalent sequences from rainbow trout and sockeye salmon (Oncorhynchus nerka) revealed a high degree of conservation. In vivo expression analysis of the sH3 promoter was carried out in both rainbow trout and zebra fish (Danio rerio) embryos. A direct comparison of the sH3 promoter with the viral RSV promoter in rainbow trout resulted in stronger expression of the sH3 promoter. Furthermore, lacZ expression directed by the sH3 promoter was ubiquitous in several different cell types in developing zebra fish embryos. These results suggest that the sH3 promoter will be useful in transgenic studies in Atlantic salmon.

AB - The histone H3 (sH3) promoter of Atlantic salmon (Salmo salar) was cloned via polymerase chain reaction using primers designed from the rainbow trout (Oncorhynchus mykiss) promoter sequence. A comparison of the nucleotide sequence with the equivalent sequences from rainbow trout and sockeye salmon (Oncorhynchus nerka) revealed a high degree of conservation. In vivo expression analysis of the sH3 promoter was carried out in both rainbow trout and zebra fish (Danio rerio) embryos. A direct comparison of the sH3 promoter with the viral RSV promoter in rainbow trout resulted in stronger expression of the sH3 promoter. Furthermore, lacZ expression directed by the sH3 promoter was ubiquitous in several different cell types in developing zebra fish embryos. These results suggest that the sH3 promoter will be useful in transgenic studies in Atlantic salmon.

UR - https://www.scopus.com/pages/publications/0032169553

M3 - Article

SN - 1053-6426

VL - 7

SP - 165

EP - 172

JO - Molecular marine biology and biotechnology

JF - Molecular marine biology and biotechnology

IS - 3

ER -

Isolation and functional analysis of the histone H3 promoter from Atlantic salmon (Salmo salar L.)

Abstract

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