Abstract
Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of the releasing cell. We isolated and characterized serum EVs of breast tumour-bearing animals, breast cancer (BC) patients, and healthy controls. EVs were characterized using transmission electron microscopy (TEM), protein quantification, western blotting, and nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed to analyse EV-miR-451a expression. Isolated EVs had the appropriate morphology and size. Patient sera contained significantly more EVs than did healthy controls. In tumour-bearing animals, a correlation between serum EV number and tumour burden was observed. There was no significant relationship between EV protein yield and EV quantity determined by NTA, highlighting the requirement for direct quantification. Using AQ-PCR to relate miRNA copy number to EV yield, a significant increase in miRNA-451a copies EV was detected in BC patient sera, suggesting potential as a novel biomarker of breast cancer.
| Original language | English (Ireland) |
|---|---|
| Article number | 766 |
| Journal | Cells |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2020 |
Keywords
- EV characterization
- biomarker
- breast cancer
- exosomes
- extracellular vesicles
- microRNA
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Moloney BM;Gilligan KE;Joyce DP;O'Neill CP;O'Brien KP;Khan S;Glynn CL;Waldron RM;Maguire CM;Holian E;Naughton E;Elhadi M;Grealish AB;Malone C;McDermott E;Dockery P;Ritter T;Prina-Mello A;Kerin MJ;Dwyer RM;
