TY - JOUR
T1 - Influence of marine sediment and divalent cations on the activity of oxytetracycline against Listonella anguillarum
AU - Smith, Peter
AU - Niland, Teresa
AU - O'Domhnaill, Fearghal
AU - O'Tuathaigh, Gearoid
AU - Hiney, Maura
PY - 1996
Y1 - 1996
N2 - Tube dilution MIC and MBC assays were used to estimate the influence of Mg++ (54 mM) Ca++ (10 mM) and 25% (w/v) marine sediment on the biological activity of oxytetracycline hydrochloride against Listonella anguillarum. Assays were performed at 22°C in phosphate-free tryptone media. MIC data indicated that, in the presence of Mg++ and Ca++, the bioactivity was 12.5 %, in the presence of sediment it was 1.5 % and when both were present it was <0.4%. Equivalent figures derived from MBC data were 6 %, < 0.4 % and < 0.4 % respectively. Concentrations in excess of 800 μg / ml oxytetracycline were required to kill the test bacteria in the presence of sediment compared to the 3 μg / ml required to have the same effect in the basic medium.
AB - Tube dilution MIC and MBC assays were used to estimate the influence of Mg++ (54 mM) Ca++ (10 mM) and 25% (w/v) marine sediment on the biological activity of oxytetracycline hydrochloride against Listonella anguillarum. Assays were performed at 22°C in phosphate-free tryptone media. MIC data indicated that, in the presence of Mg++ and Ca++, the bioactivity was 12.5 %, in the presence of sediment it was 1.5 % and when both were present it was <0.4%. Equivalent figures derived from MBC data were 6 %, < 0.4 % and < 0.4 % respectively. Concentrations in excess of 800 μg / ml oxytetracycline were required to kill the test bacteria in the presence of sediment compared to the 3 μg / ml required to have the same effect in the basic medium.
UR - http://www.scopus.com/inward/record.url?scp=0002647195&partnerID=8YFLogxK
M3 - Article
SN - 0108-0288
VL - 16
SP - 54
EP - 57
JO - Bulletin of the European Association of Fish Pathologists
JF - Bulletin of the European Association of Fish Pathologists
IS - 2
ER -