Abstract
We describe a multi-functional, cationic hyaluronic acid (HA)-based gene carrier with improved transfection over non-cross-linked HA, and negligible cytotoxicity. Cationized particles are developed by cross-linking HA chain carboxyl groups with polyamidoamine amine termini to produce well segregated particles of 350-400nm with a surface charge density of +2mV, compared with -35mV for non-cationized particles. A tethered antibody fragment retains ligand binding for cell targeting. Cationized and antibody-linked particles complex plasmid DNA efficiently and the cationized particles successfully deliver reporter genes to bovine intervertebral disk cells as an intervertebral disk regeneration model.
| Original language | English (Ireland) |
|---|---|
| Pages (from-to) | 682-690 |
| Number of pages | 9 |
| Journal | Macromolecular Bioscience |
| Volume | 15 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 May 2015 |
Keywords
- conjugated polymers
- drug delivery systems
- hyaluronic acid
- intervertebral disk
- poly(amidoamine)
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Srivastava, A,Cunningham, C,Pandit, A,Wall, JG
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