Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded dna templates

Gilles Flouriot; Caroline Pope; M.-R. Kenealy; Frank Gannon

doi:10.13025/23595

Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded dna templates

Gilles Flouriot
, Caroline Pope
, M.-R. Kenealy
, Frank Gannon

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

10 Citations (Scopus)

Abstract

Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled single-stranded DNA generated from a biotinylated single-stranded DNA template, as a long specific primer in the reverse transcription reaction. This new approach allows the detection of transcripts with a low expression level from microgram quantities of total RNA.

Original language	English (Ireland)
Journal	Nucleic Acids Research
DOIs	https://doi.org/10.13025/23595 https://doi.org/10.1093/nar/25.8.1658
Publication status	Published - 1 Apr 1997
Externally published	Yes

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10.13025/23595Licence: CC BY-NC-ND
10.1093/nar/25.8.1658

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title = "Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded dna templates",

abstract = "Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled single-stranded DNA generated from a biotinylated single-stranded DNA template, as a long specific primer in the reverse transcription reaction. This new approach allows the detection of transcripts with a low expression level from microgram quantities of total RNA.",

author = "Gilles Flouriot and Caroline Pope and M.-R. Kenealy and Frank Gannon",

year = "1997",

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doi = "10.13025/23595",

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T1 - Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded dna templates

AU - Flouriot, Gilles

AU - Pope, Caroline

AU - Kenealy, M.-R.

AU - Gannon, Frank

PY - 1997/4/1

Y1 - 1997/4/1

N2 - Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled single-stranded DNA generated from a biotinylated single-stranded DNA template, as a long specific primer in the reverse transcription reaction. This new approach allows the detection of transcripts with a low expression level from microgram quantities of total RNA.

AB - Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled single-stranded DNA generated from a biotinylated single-stranded DNA template, as a long specific primer in the reverse transcription reaction. This new approach allows the detection of transcripts with a low expression level from microgram quantities of total RNA.

UR - http://hdl.handle.net/10379/9168

U2 - 10.13025/23595

DO - 10.13025/23595

M3 - Article

SN - 0305-1048

JO - Nucleic Acids Research

JF - Nucleic Acids Research

ER -

Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded dna templates

Abstract

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